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- 详细信息
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- 技术资料
- 保质期：
1年
- 英文名：
Fructanase Mixture (purified-liquid)
- 库存：
请咨询
- 供应商：
上海经科化学科技有限公司
- CAS号：
无
- 保存条件：
-20℃
- 规格：
20,000 Units
果聚酶混合物介绍：
中文名称：果聚糖酶混合物[纯化 液体]
英文名称：Fructanase Mixture (purified-liquid)
货号：E-FRMXLQ
规格：20,000 Units
价格：4302元
类别：碳水化合物活性酶
High purity Fructanase Mixture (liquid) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
For Fructan Determination (Liquid).
Fructanase, Amyloglucosidase (E-AMGDF) and Isoamylase (E-ISAMY) are used in the enzyme hydrolysis step of the AOAC method 2001.11 for the determination of polydextrose (a low molar mass dietary fiber) in foods.
Components: exo-inulinase 2,000 U/ml (on kestose, at 40oC), endo-inulinase ˜ 100 U/ml (on fructan at 40oC), α-galactosidase < 0.08 U/ml (on polygalacturonic acid at 40oC), β-Glucanase < 0.04 U/ml (on β-glucan at 40oC) and pectinase < 0.07 U/ml (on pectin at 40oC).
Note: This product has been purified to remove α-galactosidase, β-glucanase and pectinase which interfere with the use of the preparation in the measurement of fructan (AOAC Method 997.08) and in the measurement of Polydextrose (AOAC Method 2000.11).
Fructanase and sucrase (E-SUCR) are used in are used in the enzyme hydrolysis step of the AOAC method 2016.06 and the Chinese GB Standard 5009.255-2016 for the determination of fructan in foods.
| Content: | 10 mL / 20,000 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Below -10oC |
| Formulation: | In 50% (v/v) glycerol |
| Physical Form: | Solution |
| Stability: | > 1 year under recommended storage conditions |
| Enzyme Activity: | Fructanase |
| EC Number: | endo-Inulinase: 3.2.1.7, exo-Inulinase: 3.2.1.80 |
| CAZy Family: | GH32 |
| CAS Number: | 9001-57-4, 9025-67-6, 37288-56-5 |
| Synonyms: | endo-inulinase: 1-beta-D-fructan fructanohydrolase exo-inulinase: fructan β-fructosidase |
| Source: | Asper-gillus sp. |
| Expression: | From Asper-gillus sp. |
| Specificity: | endo-Inulinase: endo-acting hydrolysis of (2,1)-β-D-fructosidic linkages in inulin. exo-Inulinase: Hydrolysis of terminal, non-reducing β-D-fructofuranoside residues in β-D-fructofuranosides. |
| Unit Definition: | endo-Inulinase: One Unit of endo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from inulin (20 mg/mL) in sodium acetate buffer (100 mM), pH 4.5. exo-Inulinase: One Unit of exo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from kestose (5 mg/mL) in sodium acetate buffer (100 mM), at pH 4.5 at 40oC. |
| Temperature Optima: | 40oC |
| pH Optima: | 4.5 |
| Method recognition: | AOAC Method 997.08, AOAC Method 2000.11, AOAC Method 2016.06, GB Standard 5009.245-2016 and GB Standard 5009.255-2016 |
果聚酶混合物说明书(PDF)请向客服索取！
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Ramirez, D. A., Altamirano, J. C. & Camargo, A. B. (2020). Food Chemistry, 337, 127648.
Nemeth, C., Andersson, A. A. M., Andersson, R., Mangelsen, E., Sun, C. & Åman, P. (2014). Food and Nutrition Sciences, 5, 581-589.
Revanna, R., Turnbull, M. H., Shaw, M. L., Wright, K. M., Butler, R. C., Jameson, P. E. & McCallum, J. A. (2013). Journal of the Science of Food and Agriculture, 93(10), 2470-2477.
Komatsu, T. R., Buriti, F. C. A., da Silva, R. C., Lobo, A. R., Colli, C., Gioielli, L. A. & Saad, S. M. I. (2013). LWT-Food Science and Technology, 50(2), 755-765.
Rakha, A., Åman, P. & Andersson, R. (2010). Food Chemistry, 119(3), 859-867.
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