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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
3年
- 英文名：
Formate dehydrogenase (C. boidinii)
- 库存：
请咨询
- 供应商：
上海经科化学科技有限公司
- CAS号：
无
- 保存条件：
常温/冷藏
- 规格：
300 Units
High purity recombinant Formate dehydrogenase (Candida boidinii) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
| Content: | 300 Units at 25oC; ~ 600 Units at 37oC |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | > 1 year under recommended storage conditions |
| Enzyme Activity: | Dehydrogenase |
| EC Number: | EC 1.2.1.2 (transferred to EC 1.17.1.9) |
| CAS Number: | 9028-85-7 |
| Synonyms: | formate dehydrogenase; formate:NAD+ oxidoreductase |
| Source: | Candida boidinii |
| Molecular Weight: | 41,331 |
| Concentration: | Supplied at ~ 75 U/mL |
| Expression: | Recombinant from Candida boidinii |
| Specificity: | Catalyses the reaction: Formate + NAD+ = CO2 + NADH |
| Specific Activity: | ~ 2 U/mg (25oC, pH 7.6 on formic acid) |
| Unit Definition: | One Unit of formate dehydrogenase is defined as the amount of enzyme required to convert one µmole of formic acid to NADH + CO2 per minute in the presence of NAD+ in potassium phosphate buffer (41 mM), pH 7.6 at 25oC. |
| Temperature Optima: | 37oC |
| pH Optima: | 7.6 |
| Application examples: | Applications for the measurement of formate in the food, fermentation, wine, beverage and dairy industries. |
如需甲酸脱氢酶[博伊丁假丝酵母]产品完整说明书，
请向上海经科客服索取！
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文献和实验1.Utilization of Cofactor Binding Energy for Enzyme Catalysis: Formate Dehydrogenase-Catalyzed Reactions of the Whole NAD Cofactor and Cofactor Pieces.
Cristobal, J. R., Nagorski, R. W. & Richard, J. P. (2023). Biochemistry, 62(15), 2314-2324.
2.Simultaneous saccharification and citric acid production from paper wastewater pretreated banana pseudostem: Optimization of fermentation medium formulation and kinetic assessment.
Laltha, M., Sewsynker-Sukai, Y. & Kana, E. G. (2022). Bioresource Technology, 361, 127700.
催化甲酸脱氢生成二氧化碳反应的酶。存在于高等植物的酶（EC1． 2． 1． 2）。以 NAD 为电子受体， HCOOH NAD → CO2 NADH H 。可为氰化物或叠氮化物所抑制。大肠杆菌等细菌的酶（ EC1． 2． 2． 1）以细胞色素 b1或泛醌（ UQ8 ）为受体，由于受含于颗粒部分的不溶性酶或氰化物等的抑制，认为是一种金属酶。此酶与甲酸的氧化酶或氢化酶偶联，与甲酸分解成氢和二氧化碳有关系。在动物的肝脏里也存在着必需 NAD 和 AMP的酶。
Enzymatic Synthesis of (S)-Phenylalanine and Related (S)-Amino Acids by Phenylalanine Dehydrogenase
substituents in the reductive amination reaction. Optically pure (S)-phe and other (S)-amino acids are quantitatively synthesized from their oxo-analogs using PheDH, with a regeneration of NADH by formate dehydrogenase from Candida boidinii No. 2201. Acetone
【资源】全面归类网址：毕赤酵母表达各种来源蛋白（细菌，真菌，植物，人类等）
giganteus a-sarcin ribotoxinS, 1 mg/L, synthetic native,PHO1[77]Aspergillus niger β-glucosidaseS, 0.5 g/L, PHO1313Aspergillus niger phytase (phyA)S, 65 U/ml, α-MF[78]Aspergillus niger endo-β-1,4-xylanaseS, 60 mg/L, SUC2, native311Barley glucosidase345Candida
