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- 2025年12月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
＞1年
- 英文名：
Citric Acid
- 库存：
大量
- 供应商：
上海经科化学科技有限公司
- 规格：
72 assays
柠檬酸检测试剂盒，原装进口！
| Content: | 72 assays (manual) / 720 assays (microplate) / 840 assays (auto-analyser) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | Citric Acid |
| Assay Format: | Spectrophotometer, Microplate, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Decrease |
| Linear Range: | 1.0 to 100 µg of citric acid per assay |
| Limit of Detection: | 0.491 mg/L |
| Reaction Time (min): | ~ 5 min |
| Application examples: | Grape juice, wine, beer, fruit juices, soft drinks, tea, dairy products (e.g. cheese), meat, processed meat, vegetable and fruit products, bakery products, paper, pharmaceuticals, cosmetics and other materials (e.g. biological cultures, samples, etc.). |
| Method recognition: | Methods based on this principle have been accepted by MEBAK, OIV, EU, ISO2963, AOAC and IFU22 (NOTE: If the enzyme oxaloacetate decarboxylase is present in the sample, some of the oxaloacetate product is converted to pyruvate. Therefore, to ensure citric acid is measured quantitatively, D-lactate dehydrogenase (D-LDH) is employed to efficiently convert any pyruvate produced into D-lactate and NAD+). |
- Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
- Reconstituted citrate lyase stable for 4 weeks at 4oC / 6 months below -10oC
- Buffer / cofactor / enzyme tablets for efficient use of kit components
- PVP incorporated to prevent tannin inhibition
- Very competitive price (cost per test)
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
- Suitable for manual, microplate and auto-analyser formats
The Citric Acid test kit is a flexible and simple method for the rapid and reliable measurement and analysis of citric acid (citrate) in foods, beverages and other materials.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
View our full list of organic acid test kits.
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文献和实验1.Physicochemical changes during controlled laboratory fermentation of cocoa (CCN-51) with the inclusion of fruits and on-farm inoculation.
Peña González, M. A., Ortiz Urgiles, J. P., Santander Pérez, F. A., Lazo Vélez, M. A. & Caroca Cáceres, R. S. (2023). Brazilian Journal of Food Technology, 26, e2023013.
2.Simultaneous saccharification and citric acid production from paper wastewater pretreated banana pseudostem: Optimization of fermentation medium formulation and kinetic assessment.
Laltha, M., Sewsynker-Sukai, Y. & Kana, E. G. (2022). Bioresource Technology, 361, 127700.
柠檬酸发酵 citric-acid fermentation
属于氧化发酵形式之一，即从糖经过氧化而产生柠檬酸。最有名的生产菌是黑曲霉。其生产方法有以淀粉渣作基质的固体培养法和以糖液为基质的液体培养法。后者是根据环境条件所采用的适宜方法，如使用浅盘或转筒的表面培养法；依靠通气搅拌的深层培养法等。目前采用深层培养法进行生产的较多。关于柠檬酸的生成机制，一般认为由糖质产生的丙酮酸一部分，变成乙酰辅酶 A和另一部分与二氧化碳结合成草酰乙酸之后，通过柠檬酸合酶（ synthase）的作用而生成柠檬酸。
无色结晶，作为柠檬酸循环之一在好氧代谢中起着重要的作用。广泛分布于生物界，此外作为细菌、霉菌的发酵生成物而大量产生。是Ca离子的捕获剂，其钠盐（ sodium citrate）可用作血液的抗凝剂。在柠檬、桔子等有酸味的果实中以游离状态存在。在线粒体膜上有柠檬酸的运输系统。柠檬酸还对磷酸果糖激酶作为效应物而起抑制作用。
的起始物质。因为此循环周转的强度依赖于其所含物质的量，所以必须补给转入各合成途径的物质的消耗，丙酮酸羧化酶或乙醛酸循环与此途径有关。柠檬酸循环局限在线粒体内的可溶部分（基质matrix）。循环由以下八个步骤组成：（1）草酰乙酸与乙酰CoA的缩合→柠檬酸，（2）柠檬酸的脱水加水→异柠檬酸，（3）异柠檬酸的氧化脱羧→α－酮戊二酸，（4）α－酮戊二酸的氧化脱羧与CoA的参入→琥珀酰CoA，（5）琥珀酰CoA与Pi，GDP的反应→琥珀酸、GTP、CoA，（6）琥珀酸脱氢→延胡索酸，（7）延胡索酸加水→L－
