Recombinant Human Fc γ RI/FCGR1A/CD64 (C-6His) (CM60)
产品说明(Description)
Recombinant Human High Affinity Immunoglobulin Gamma Fc Receptor I is produced by our Mammalian expression system and the target gene encoding Gln16-Pro288 is expressed with a 6His tag at the C-terminus.
Accession #: P12314
Known as: Ig gamma-4 chain C region,IgG4 Fc
制剂(Formulation)
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
质量控制(Quality Control)
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Bioactivity: Immobilized Human SIgG1-Fc (Cat#CX82) at 2μg/ml (100 μl/well) can bind Human CD64-His(Cat#CM03).
The ED50 of Human CD64-His(Cat#CM60) is 0.05ug/ml .
Bioactivity-BLI:
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Loaded Human CD64-His(Cat#CM60) on HIS1K Biosensor, can bind Anti-Human HER2 mAb-His(Cat#A062) with an affinity constant of 0.183nM as determined in BLI assay. |
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Loaded Human CD64-His(Cat#CM60) on HIS1K Biosensor, can bind Anti-Human HER2 mAb-His(Cat#A062) with an affinity constant of 0.183nM as determined in BLI assay. |
复溶(Reconstitution)
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存(Storage)
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
背景(Background)
CD64 (FcγRI), one of the Fc receptors for IgG, is a membrane glycoprotein that mediates endocytosis, phagocytosis, antibody-dependent cellular cytotoxicity, cytokine release, and superoxide production. CD64 is also structurally distinct, containing an extracellular Ig-interactive region of three Ig-like domains in contrast to the two domains of the low affinity receptors FcγRII and FcγRIII. It is normally expressed on the surfaces of monocytes and macrophages.
电泳(SDS-PAGE)
FOR RESEARCH USE ONLY