MRE-269

利用共聚焦显微镜实现 DNA 修复蛋白可视化

处（包括双链 DNA 断裂位点）的募集过程。采集的图像不仅帮助我们确定募集到断裂位点修复蛋白的动力学信息和聚集水平，还验证了内源转录调节因子和 DDR 通路因子在 DNA 断裂位点的共定位。   图 1：实验方案示意图   将 MRE11 cDNA 克隆到带有 GFP 标签的表达载体中，然后将其转染到 U2OS 细胞。在使用溴脱氧尿嘧啶核苷（BrdU）或 Hoechst 进行标记后，使用 FV3000 共聚焦显微镜的 405 nm 激光对核内目标区域（ROI）进行线扫描诱导 DNA 损伤。随后使用

Analysis of ProteinDNA Interactions During Meiosis by Quantitative Chromatin Immunoprecipitation (qChIP)

at defined binding sites in vivo. Here we use qChIP to study Mre11 binding to three chromosomal loci during meiosis. We show that Mre11 interacts with a known hotspot region (YCR048 ) in the R-band of chromosome III, but not with a cold region in the G-band

Preparation of Tonsil Lysate

cllect frm mre than ne surce. Nte 2: Yu can prep each sample as yu receive it r wait until yu have a ~ 50g f tnsils. If yu decide t prep them right away then perfrm steps 2 t 4 and then freeze at -80℃. If yu want t wait until yu have ~50g f tnsil,yu