- ￥5820
- 义翘神州
- 40589-V08H8
- 北京
- 2026年01月26日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
99
- 英文名：
SARS-CoV-2 Spike S1+S2 (D614G) trimer Protein (ECD, His tag)
- 保质期：
12个月
- 供应商：
北京义翘神州科技股份有限公司
- 保存条件：
-20℃ to -80℃
- 规格：
100 µg
纯度> 90 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.
内毒素< 1.0 EU per μg protein as determined by the LAL method.
生物活性Immobilized human ACE2 protein (mFc tag)(Cat:10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike S1+S2 (D614G) trimer Protein (ECD, His tag)(Cat:40589-V08H8),the EC50 of SARS-CoV-2 Spike S1+S2 (D614G) trimer Protein (ECD, His tag)(Cat:40589-V08H8) is 4-24 ng/mL.
蛋白构建A DNA sequence encoding the SARS-CoV-2 Spike S1+S2 (YP_009724390.1, with mutations D614G, F817P, A892P, A899P, A942P, K986P, V987P and furin cleavage site mutants) expressed with the bacteriophage T4 fibritin and a polyhistidine tag at the C-terminus.
表达宿主HEK293 Cells
种属SARS-CoV-2
预测 N 端Val 16
分子量The recombinant SARS-CoV-2 Spike S1+S2 consists of 1231 amino acids and predicts a molecular mass of 136.47 kDa.
缓冲液Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
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文献和实验2, Tang, J; et al. Respiratory mucosal immunity against SARS-CoV-2 following mRNA vaccination. Science immunology, PMID: 35857583
3, Adams, LE; et al. Fc-mediated pan-sarbecovirus protection after alphavirus vector vaccination. Cell reports, PMID: 37000623
4, Hornsby, H; et al. Omicron infection following vaccination enhances a broad spectrum of immune responses dependent on infection history. Nature communications, PMID: 37604803
5, Sui, Y; et al. Adjuvanted subunit intranasal vaccine prevents SARS-CoV-2 onward transmission in hamsters. bioRxiv : the preprint server for biology, PMID: 38798555
6, Han, X; et al. Fast and facile synthesis of amidine-incorporated degradable lipids for versatile mRNA delivery in vivo. Nature chemistry, PMID: 38982196
7, Han, X; et al. Optimization of the activity and biodegradability of ionizable lipids for mRNA delivery via directed chemical evolution. Nature biomedical engineering, PMID: 39578640
8, Blizard, GS; et al. Monitoring mRNA vaccine antigen expression in vivo using PET/CT. Nature communications, PMID: 40044669
总蛋白（包括质膜和细胞器膜蛋白），但费时费力。目前，有一种最新型有效的膜蛋白提取工具，并具有高纯度、高特异性、高载量温和提取的优点。是基于1980年科学家在牛眼视网膜中发现的一种命名为Rho1D4的氨基酸序列以及其对应的抗体来作为亲和标签纯化膜蛋白取得了非常好的效果，将Rho1D4抗体链接在琼脂糖或磁珠上，用于亲和层析带有Rho1D4标签的膜蛋白，能近乎完美的解决这些研究难题。1） 什么是Rho1D4?Rho1D4是指在牛眼视网膜细胞内的牛视紫红质C端的最后九位氨基酸。Rho1D4得名于与该序列
后继的亲和纯化。目前最为流行的融合标签自然是 His-Tag，和 GST，来自 G-Biosciences 等公司都有质量非常稳定可靠的产品，种类还特别丰富，分别对应不同的上样量和工作压力和流速的需要。笔者在这里介绍一下江湖中盛传的 Tag 蛋白纯化的「刀剑笑」，目的就是为在产品选择上帮你省点事...... 「横刀」-蛋白亲和纯化 GST 树脂 G-Biosciences 的谷胱甘肽树脂，用于分离 GST 重组蛋白 用来亲和纯化带有 GST 标签的蛋白。树脂由还原性的谷胱甘肽通过 10
。 2019 年 4 月 Thomas G Fazzio 教授等发表在 Cell 期刊的 uliCUT&RUN 技术，将蛋白质-DNA 互作研究升级到单细胞水平。近几个月来，又相继有文章报道了 CUT&Tag 等技术，进一步优化了蛋白质-DNA 互作研究方法，本文将对系列方法一并总结介绍。 1.蛋白质-DNA 互作新技术发展历程 1997 年 Orlando等研发出ChIP[1]，主要用于研究转录因子与 DNA 结合位点的序列信息。 2009 年 Dominic Schmidt
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