Recombinant Human TSLP (R127A,

Recombinant Human TSLP (R127A, R130A, C-10His) (C04R)

 

产品说明(Description)

Recombinant Human Thymic stromal lymphopoietin is produced by our Mammalian expression system and the target gene encoding Tyr29-Gln159(R127A, R130A) is expressed with a 10His tag at the C-terminus.

Accession #: Q969D9

Known as: Thymic stromal lymphopoietin; TSLP

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

 

质量控制(Quality Control)

Purity:           Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

Bioactivity-BLI:		Bioactivity-ELISA:
Loaded Anti-Human TSLP mAb-Fc(Cat#NC066) on Protein A Biosensor, can bind Human TSLP-His(Cat#C04R) with an affinity constant of <10-3 nM as determined in BLI assay.		Immobilized Anti-Human TSLP mAb(Cat#NC066) at 2μg/ml (100 μl/well)can bind Human TSLP-His(Cat#C04R). The ED50 of  Human TSLP-His(Cat#C04R)is 4.48 ng/ml.

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.

Reconstituted protein solution can be stored at 4-7°C for 2-7 days.

Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景(Background)

Thymic stromal lymphopoietin (TSLP) is a novel member of the hemopoietic cytokine family that promotes the development of B cells and shares overlapping activity with IL-7. The human TSLP protein comprises a 28 amino acids (aa) signal sequence and 131 aa mature region. Human TSLP has two isoforms lfTSLP and sfTSLP produced by alternative splicing . lfTSLP is expressed in a number of tissues including heart, liver and prostate, and sfTSLP (63aa) is predominantly expressed in keratinocytes of oral mucosa, skin and in salivary glands. In aa sequence level, Human TSLP displays about 43% identity with mouse TSLP.TSLP is a cytokine that functions mainly on myeloid cells; it induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells.TSLP has proliferative effects on the myeloid cell line and may initiate asthma or atopic dermatitis responses by directly activating mast cells . TSLP signals cells via the interleukin-7 receptor-α chain (IL-7Rα),shared with IL-7, together with the TSLP receptor (TSLPR) subunit. Recent studies indicate that TSLP and its receptor are novel therapeutic targets for rheumatoid arthritis,for increased intraarticular TSLP concentrations in patients has caused chemotaxis and activation of arthritogenic T cells.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY