Chymostatin (9076-44-2) is a potent, competitive, slow-binding inhibitor of a-, b-, g-, d-chymotrypsin, papain and cathepsins B/G (chymotryptase-like serine proteases)1, Ki=9.36 and 13.1 nM for chymotrypsin and chymase2. Cathepsin G Ki=0.15 mM.3 Consists of a mixture of type A (L-Leu), B (L-Val) and C (L-Ile) forms.1 Decreases plasma and tissue levels of angiotensin II without lowering mean blood pressure in a hypertensive rat model.4 Commonly used in lysis buffers to prevent degradation of proteins. Typical working concentration is 6-60 µg/ml. 凝乳抑制素凝乳抑制素(9076-44-2)是a-, b-, g-, d-糜蛋白酶,木瓜蛋白酶和组织蛋白酶b / g(糜蛋白酶样丝氨酸蛋白酶)1的一种有效的竞争性的慢结合抑制剂,对糜蛋白酶和糜蛋白酶2,Ki=9.36和13.1 nM。 由a型(L-Leu)、B型(L-Val)和C型(L-Ile)的混合物组成 降低血浆和组织中血管紧张素II的水平,但不降低高血压大鼠模型的平均血压 通常用于裂解缓冲液,以防止蛋白质降解。 典型工作浓度为6-60µg/ml。
References/Citations:
1) Umezawa et al. (1970), Chymostatin, a new chymotrypsin inhibitor produced by actinomycetes; J. Antibiot. (Tokyo) 23 425 2) Johnson et al. (1998), Inactivation of chymotrypsin and human skin chymase: kinetics of time-dependent inhibition in the presence of substrate; Biochim. Biophys. Acta 953 269 3) Stein and Strimpler (1987), Slow-binding of chymotrypsin and cathepsin G by the peptide aldehyde chymostatin; Biochemistry 26 2611 4) Roszkowska-Chojecka et al. (2015), Effects of chymostatin, a chymase inhibitor, on blood pressure, plasma and tissue angiotensin II, renal haemodynamics and renal excretion in two models of hypertension in the rat; Exp. Physiol. 100 1093
CAS:
9076-44-2
Catalog Number:
10-2722
Activity:
Inhibitor of chymotrypsin-like serine proteases
Chemical Name:
[(S)-1-Carboxy-2-phenylethyl]-carbamoyl-a-[2-amidohexahydro-4(S)-pyrimidyl]-(S)-glycyl-[A= Leu, B=Val, C=Ile]-phenylalaninal
Molecular Weight:
605.0 (ave.)
Molecular Formula:
C31H41N7O6 Solubility:
Soluble in DMSO (up to 20 mg/ml)
Physical Properties:
White or off-white solid
Purity:
96% by TLC
NMR (Conforms)
Storage Temperature:
-20°C
Stability:
Stable for 1 year from date of purchase as supplied. Solutions in DMSO may be stored at -20°C for up to 1 month.
Shipping Code:
RT
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