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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
胰高血糖素样肽1多肽
- 抗体英文名:
GLP-1
- 靶点:
细胞浆 细胞膜 分泌型蛋白
- 浓度:
1mg/ml
- 应用范围:
Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- 宿主:
Rabbit
- 适应物种:
Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
- 保质期:
一年
- 抗原来源:
Rabbit
- 目录编号:
TF21166R
- 级别:
I级
- 库存:
10
- 供应商:
晶风生物
- 标记物:
FITC/Alexa/CY357/BIo/HRP
- 克隆性:
Polyclonal
- 保存条件:
-20
- 形态:
Liquid
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived
- 规格:
100ug
产品规格:100ul/200ul(部分有50ul,如需更大包装或其他具体规格,请咨询客服)
研究领域:肿瘤 细胞生物 免疫学等
抗体来源:Rabbit
克隆类型:Polyclonal
交叉反应:Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,
产品应用:WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状:Liquid
浓 度:1mg/ml
免 疫 原:KLH conjugated synthetic peptide derived
亚 型:IgG
纯化方法:affinity purified by Protein A
储 存 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
胰高血糖素样肽1多肽相关抗体示例(非本抗体,如需本抗体,请联系客服索要说明书):
Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.

paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.

胰高血糖素样肽1多肽
Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Tissue/cell: rat colitis tissue; paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB staining
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文献和实验采用模型系统进行研究,可以得到对蛋白质的立体化学有贡献的实质性理解。合成多肽为确定与蛋白质的结构和功能有关的构象及相互作用,提供了颇具吸引力的途径。 螺旋结构是蛋白质、核酸、多糖及许多合成多肽的共同结构特征。研究模型多肽在固态和溶液中的螺旋结构,对明确解释在纤维蛋白和球蛋白的构象研究中得出的结论具有重要意义。 多肽是由α氨基酸按下面的通式组成的聚酰胺,其中R是α氨基酸中的侧链: 根据下面的基本假定,Pauling和Corey设想α-螺旋是折叠多肽比较稳定的二级结构之一: 多肽残基是反式共
保存: 大多肽在-20℃很稳定,特别是冷冻干燥并保存在干燥器中,在将它们暴露于空气之前, 冷冻干燥多肽可以放于室温。这将是湿度影响减少,当无法冷冻干燥时,最好的方法是以小的工作样量存放。 对于含Cys, Met orTrP的多肽,脱氧缓冲剂对其溶解必不可少,因为这种多肽可易空气氧化, 在封瓶前,慢慢流过多肽的氮气或氩气也会降低氧化作用。含Gln或Asn的多肽也容易降解,所有这些肽与不含这些有问题解苷的那些肽相比,生命期有限。 溶解性: 大多肽的道选溶剂是超纯
指许多氨基酸单位用肽键互相连接构成的长链。肽和蛋白质没有严格的界限,一般把只含有数十个或更少个氨基酸单位的多肽叫做肽,把氨基酸单位比较多的多肽叫蛋白质。常把多肽中的氨基酸单位叫做氨基酸残基,因为这些单位在互相连接时已失去分子的一部分,而不是完整的氨基酸了。只有肽链两端的氨基酸残基才含有自由的α -氨基或自由的α -羧基。有自由α -氨基的末端残基叫做氨基末端(或 N端)残基,有自由α -羧基的末端残基叫做羧基末端(或 C端)残基。给多肽命名时按照从 N端到 C端残基的顺序
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