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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
心房钠尿因子/心钠素/心房利钠肽抗体
- 抗体英文名:
Atrial natriuretic factor
- 靶点:
细胞浆 细胞膜 分泌型蛋白
- 浓度:
1mg/ml
- 应用范围:
Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- 宿主:
Rabbit
- 适应物种:
Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
- 保质期:
一年
- 抗原来源:
Rabbit
- 目录编号:
TF2040R
- 级别:
I级
- 库存:
10
- 供应商:
晶风生物
- 标记物:
FITC/Alexa/CY357/BIo/HRP
- 克隆性:
Polyclonal
- 保存条件:
-20
- 形态:
Liquid
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived
- 规格:
50ul/100ul/200ul
产品名称:Atrialnatriureticfactor心房钠尿因子/心钠素/心房利钠肽抗体
产品规格:100ul/200ul(部分有50ul,如需更大包装或其他具体规格,请咨询客服)
研究领域:肿瘤 细胞生物 免疫学等
抗体来源:Rabbit
克隆类型:Polyclonal
交叉反应:Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,
产品应用:WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状:Liquid
浓 度:1mg/ml
免 疫 原:KLH conjugated synthetic peptide derived
亚 型:IgG
纯化方法:affinity purified by Protein A
储 存 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Atrialnatriureticfactor心房钠尿因子/心钠素/心房利钠肽抗体相关抗体示例(非本抗体,如需本抗体,请联系客服索要说明书):
Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.
paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.
Atrialnatriureticfactor心房钠尿因子/心钠素/心房利钠肽抗体
Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Tissue/cell: rat colitis tissue; paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB staining
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文献和实验尿多肽(atrial natriuretic polypeptide),简称心钠素,有很强的利尿、排钠、扩张血管和降血压作用。近年研究还证明,心肌还能分泌其它多种生物活性物质,如与心钠素作用相似的脑钠素、抗心律失常肽和内源性洋地黄素(又称内洋地黄素)。心肌细胞还具有合成肾素和血管紧张素的能力,对促进心肌细胞生长,增强心肌收缩力等有重要作用。 在心房肌和心室肌之间,有由致密结缔组织组成的支持性结构,构成心脏的支架,也是心肌和心瓣膜的附着处,称心骨骼(cardiac skeleton
元素,但硒过多也会对人体产生毒性作用,如脱发、指甲脱落、周围性神经炎、生长迟缓及生育力降低等。因此不可盲目补硒、推荐的补硒安全值为400-500μg/d。 六、氟 在人体内氟含量约为2-3g,其中90%积存于骨及牙中。每日需要量为2.4mg。 氟主要经胃部吸收,氟易吸收且吸收较迅速。氟主要经尿和粪便排泄、体内氟约80%从尿排出。 氟能与羟磷灰石吸附,取代其羟基形成氟磷灰石,能加强对龋齿的抵抗作用。 3(Ca3(PO4)2)・Ca(OH)2+2F-→3(Ca3(PO
血清冻干粉 标准品 143 IU / ampoule 阿朴脂蛋白A-1 冻干粉 标准品 1.66mg / vial 阿朴脂蛋白B冻结品 标准品 1.22g / l. Vials. 1 ml. 精氨酸抗利尿激素冻干粉 标准品 8.2 IU / ampoule. 人心房利钠尿因子冻干粉 标准品 2.5 IU / ampoule. 第三页 枯草杆菌抗生素冻干粉 标准品 Approximately 100mg of zinc bacitracin (74IU/ mg) 碱性成纤维细胞生长因子 (bFGF
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