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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
副肿瘤性神经元抗原抗体
- 抗体英文名:
MA2
- 靶点:
细胞浆 细胞膜 分泌型蛋白
- 浓度:
1mg/ml
- 应用范围:
Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- 宿主:
Rabbit
- 适应物种:
Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
- 保质期:
一年
- 抗原来源:
Rabbit
- 目录编号:
TF18560R
- 级别:
I级
- 库存:
10
- 供应商:
晶风生物
- 标记物:
FITC/Alexa/CY357/BIo/HRP
- 克隆性:
Polyclonal
- 保存条件:
-20
- 形态:
Liquid
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived
- 规格:
50ul/100ul/200ul
产品规格:100ul/200ul(部分有50ul,如需更大包装或其他具体规格,请咨询客服)
研究领域:肿瘤 细胞生物 免疫学等
抗体来源:Rabbit
克隆类型:Polyclonal
交叉反应:Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,
产品应用:WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状:Liquid
浓 度:1mg/ml
免 疫 原:KLH conjugated synthetic peptide derived
亚 型:IgG
纯化方法:affinity purified by Protein A
储 存 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
MA2副肿瘤性神经元抗原抗体相关抗体示例(非本抗体,如需本抗体,请联系客服索要说明书):
Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.

paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.

MA2副肿瘤性神经元抗原抗体
Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Tissue/cell: rat colitis tissue; paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB staining
欢迎新老客户咨询订购:MA2副肿瘤性神经元抗原抗体
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文献和实验指标提高一倍多,往往可以获得一些附加的更特异性的重要线索,甚至能够挽救很多患者的生命。 副瘤性综合征 副瘤性综合征(PNS)是神经系统症状伴随恶性肿瘤,它是一种在癌症未转移或未浸润情况下的远隔效应。自身抗体的检测可以帮助诊断PNS,并提供潜在肿瘤的第一个证据。几乎每种PNS疾病都可发现一个以上的神经元自身抗体;反之,每个抗体也可在不同PNS疾病中体现。有数据显示,大约30%的PNS患者可以检测到多个神经元自身抗体。例如,在副瘤性边缘性脑炎中常见抗Hu、Ma2、CV
有关。 ⑤当MHC-Ⅱ类分子表达时获得抗原提呈功能。如在巴金森氏病及 老年性痴呆 症的病灶中有HLA-DR阳性小胶质细胞的分布。 3.脑内免疫反应的特点 脑内不但有星形细胞和小胶质细胞等免疫辅助细胞,还存在内源性抗炎机制。因此,中枢神经系统一方面不是完全的免疫特许部位,另一方面脑内的免疫反应经常受抑制或下行性调节。 (二)外周神经系统 交感神经节中的肾上腺素能神经元在交感神节去传入后或离体培养时,胞体中SP及编码SP的PPt mRNA
网络 ICC的几种特殊应用 近年 ICC技术在下述方面的应用,令人注目,有着广阔的前景。现介绍如下: 一、ICC在铺片中应用 铺片(Whole Mount Stretch Preparation) 是研究神经分布应用较早、较广的方法。19世纪末,Dogiel和Cajal等利用铺片技术,结合镀银及甲基蓝染色,观察肠道神经丛模式、神经元种类及其相关联的间质细胞。近年来,人们进一步认识
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