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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
骨骼肌细胞线粒体膜蛋白PARL抗体
- 抗体英文名:
PARL
- 靶点:
细胞浆 细胞膜 分泌型蛋白
- 浓度:
1mg/ml
- 应用范围:
Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- 宿主:
Rabbit
- 适应物种:
Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
- 保质期:
一年
- 抗原来源:
Rabbit
- 目录编号:
TF7634R
- 级别:
I级
- 库存:
10
- 供应商:
晶风生物
- 标记物:
FITC/Alexa/CY357/BIo/HRP
- 克隆性:
Polyclonal
- 保存条件:
-20
- 形态:
Liquid
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived
- 规格:
50ul/100ul/200ul
产品规格:100ul/200ul(部分有50ul,如需更大包装或其他具体规格,请咨询客服)
研究领域:肿瘤 细胞生物 免疫学等
抗体来源:Rabbit
克隆类型:Polyclonal
交叉反应:Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,
产品应用:WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状:Liquid
浓 度:1mg/ml
免 疫 原:KLH conjugated synthetic peptide derived
亚 型:IgG
纯化方法:affinity purified by Protein A
储 存 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PARL骨骼肌细胞线粒体膜蛋白PARL抗体相关抗体示例(非本抗体,如需本抗体,请联系客服索要说明书):
Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.

paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.

PARL骨骼肌细胞线粒体膜蛋白PARL抗体
Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Tissue/cell: rat colitis tissue; paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB staining
欢迎新老客户咨询订购:PARL骨骼肌细胞线粒体膜蛋白PARL抗体
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文献和实验取出所需分装管,并在规定时间内使用完毕,避免整管长时间暴露于室温。 4. 机械解离法 vs 酶解法 vs 组合法? 解离方法各有优缺点,根据不同组织类型和需求选择合适的解离方式。 (1)机械解离法:通过剪切、研磨、吹打等物理力破坏细胞间连接,无需酶试剂无需孵育时间成本较低,但是容易造成细胞损伤(如碎片多活率低)和解离不完全(如致密组织),常用于快速解离脾脏等柔软组织。 (2)酶解法:利用蛋白酶(胶原酶、胰酶、木瓜蛋白酶等)降解细胞外基质,温和解离保护细胞膜完整性,但是易破坏表位,且需要挑选合适
的抗原或抗体及其酶结合物等免疫测定试剂几成为现实的新一代试剂,各种新型使用的测定方法也不断出现。 HBsAg试剂特点(检测模式:临 床抗体夹心法): 包被抗体为山羊多抗,多抗具有高亲和性和对抗原各种表位的反应性。 酶表抗体为与HBsAg有不同结合位点的鼠复合单位。 可测得HBsAg变异样品。 提高检测的特异性(99.98%) 提高检测的灵敏度,应用Parl Ehrich 学说(PEI)HBsAg标准品,对ad标准品的灵敏度为0.05ng/ml对ay标准品灵敏度为0.025ng/ml
排列。当细胞融合80%以上后,开始形成肌管。2、免疫细胞化学染色1:肌卫星细胞胞浆中含有结蛋白(desmin),可采用鼠抗人及小鼠desmin一抗对卫星细胞进行免疫染色鉴定。3、免疫细胞化学染色2:采用骨骼肌特异性的肌球蛋白(myosin)单克隆抗体检测。取含骨骼肌细胞盖玻片常规处理后进行myosin的细胞免疫化学染色,PBS代替一抗做阴性对照。结果判定:以细胞浆出现棕黄色颗粒为阳性染色。六、注意事项1、胰酶消化过程中,也可采用15min两步消化的方法进行。根据所取组织个体年龄决定,一般成年大小
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