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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
钙激活钾通道蛋白 α 1抗体
- 抗体英文名:
KCNMA1/BK channel
- 靶点:
细胞浆 细胞膜 分泌型蛋白
- 浓度:
1mg/ml
- 应用范围:
Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- 宿主:
Rabbit
- 适应物种:
Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
- 保质期:
一年
- 抗原来源:
Rabbit
- 目录编号:
TF0729R
- 级别:
I级
- 库存:
10
- 供应商:
晶风生物
- 标记物:
FITC/Alexa/CY357/BIo/HRP
- 克隆性:
Polyclonal
- 保存条件:
-20
- 形态:
Liquid
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived
- 规格:
50ul/100ul/200ul
产品规格:100ul/200ul(部分有50ul,如需更大包装或其他具体规格,请咨询客服)
研究领域:肿瘤 细胞生物 免疫学等
抗体来源:Rabbit
克隆类型:Polyclonal
交叉反应:Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,
产品应用:WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状:Liquid
浓 度:1mg/ml
免 疫 原:KLH conjugated synthetic peptide derived
亚 型:IgG
纯化方法:affinity purified by Protein A
储 存 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
KCNMA1/BKchannel钙激活钾通道蛋白α1抗体相关抗体示例(非本抗体,如需本抗体,请联系客服索要说明书):
Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.

paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.

KCNMA1/BKchannel钙激活钾通道蛋白α1抗体
Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Tissue/cell: rat colitis tissue; paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB staining
欢迎新老客户咨询订购:KCNMA1/BKchannel钙激活钾通道蛋白α1抗体
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文献和实验由三个重复部分构成的环形分子,能顺浓度梯度转运K+。DNP和FCCP可转运H+。 通道离子载体( channel former ) : 如短杆菌肽A(granmicidin),是由15个疏水氨基酸构成的短肽,2分子形成一个跨膜通道,有选择的使单价阳离子如H+、Na+、K+按化学梯度通过膜。 (二)通道蛋白(channel protein) 是跨膜的亲水性通道,允许适当大小的离子顺浓度梯度通过,故又称离子通道。 有些通道蛋白长期开放,如钾泄漏通道; 有些通道蛋白平时处于关闭状态,仅在特定刺激
、抗Sm抗体 D、抗SS-DNA抗体 E、抗J0一1抗体 【正确答案】 B 【答案解析】 33、 人感染日本血吸虫产生的免疫为 A、带虫免疫 B、伴随免疫 C、体液免疫 D、细胞免疫 E、以上都不是 【正确答案】 B 【答案解析】 34、 引起幼儿脑膜炎的流感嗜血杆菌以何型多见 A、a型 B、c型 C、f型 D、b型 E、e型 【正确答案】 D 【答案解析】 35、 急性
分子上的“工厂”。一项精妙的超微结构研究表明,当在颗粒样吞噬细胞发生作用时,ER膜向下形成吞噬杯。这个模型引发了MHCⅠ提呈机制与被吞噬的微生物相接近。两个随后研究的建立补充了这个机制。在吞噬体膜上存在蛋白酶、TAP、热休克蛋白HSP-70,钙联接蛋白和钙网蛋白是必须的。只要当抗原是吞噬细胞时,它激活CD8+T细胞。胞饮作用(例如,可溶性物质吸收)不能激活CD8+T细胞。这个模型给以往的研究提供一个解释,特定的抗原如细菌,通过交叉提呈诱导CD8+T细胞。 交叉启动 尽管沙门
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