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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
多配体聚糖4抗体
- 抗体英文名:
Syndecan 4
- 靶点:
细胞浆 细胞膜 分泌型蛋白
- 浓度:
1mg/ml
- 应用范围:
Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- 宿主:
Rabbit
- 适应物种:
Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
- 保质期:
一年
- 抗原来源:
Rabbit
- 目录编号:
TF2926R
- 级别:
I级
- 库存:
10
- 供应商:
晶风生物
- 标记物:
FITC/Alexa/CY357/BIo/HRP
- 克隆性:
Polyclonal
- 保存条件:
-20
- 形态:
Liquid
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived
- 规格:
50ul/100ul/200ul
产品规格:100ul/200ul(部分有50ul,如需更大包装或其他具体规格,请咨询客服)
研究领域:肿瘤 细胞生物 免疫学等
抗体来源:Rabbit
克隆类型:Polyclonal
交叉反应:Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,
产品应用:WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状:Liquid
浓 度:1mg/ml
免 疫 原:KLH conjugated synthetic peptide derived
亚 型:IgG
纯化方法:affinity purified by Protein A
储 存 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Syndecan4多配体聚糖4抗体相关抗体示例(非本抗体,如需本抗体,请联系客服索要说明书):
Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.

paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.

Syndecan4多配体聚糖4抗体
Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Tissue/cell: rat colitis tissue; paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB staining
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文献和实验单链抗体可变区基因片段五聚体作用于凋亡诱导配体受体-2来增强其对癌症的治疗作用
诱导配体(TRAIL或载脂蛋白2配体)已经被用作一个有效的癌症治疗靶点。TRAIL是肿瘤坏死因子家族的一员。通过形成三聚物,将它的受体聚集起来,TRAIL受体1(死亡受体(DR)4)和/或TRAIL受体2(DR 5),通过caspase级联反应引起的外源途径来诱导凋亡。由于TRAIL对非转化细胞几乎没有细胞毒性,它要优于肿瘤坏死因子或CD95。这一优势使得TRAIL成为一个癌症治疗的理想的靶点。的确,TRAIL或针对DR4和DR5的单克隆激动性抗体目前正活跃于I/II期的临床试验,并显示了很好的癌
疾病的复发,所以研发出能够靶向骨髓瘤细胞并产生良好预后效果的临床药物仍很有必要。 多聚体蛋白聚糖(syndecan-1, CD138)属于粘附分子整合素跨膜粘结蛋白聚糖家族成员,它主要分布于内皮细胞、上皮细胞、纤维母细胞、角质细胞表面,通过其分子表面的硫酸肝素侧链可结合一系列配基。如细胞粘附分子、基质成分、生长因子、酶和酶抑制物等,以共受体(cell surface co-receptors)方式调节细胞与微环境之间的相互作用,参与组织器官分化发育、血管形成、组织再生等一系列生理过程的调节
- Streps 抗体具有高度特异性,按照功能划分的抗体有 Fab 区和 Fc 区。其中的 Fab 是抗原结合片段,对特异性抗原有亲和力(affinity)。 如果避免使用高亲和力抗体,可以考虑选取抗体上的 Fab 片段。将 Fab 与亲和 Strep 标签融合后,得到的表位标签Fab - Streps,留有抗体-抗原相互作用,还具备标签-配体相互作用。 Fab - Streps 的 Fab 段既对目的细胞的特异性 CD 标记有着亲和力,能抓取目的细胞;Strep 段又能结合亲和
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