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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
沉默调节相关蛋白6抗体
- 抗体英文名:
SIRT6
- 靶点:
细胞浆 细胞膜 分泌型蛋白
- 浓度:
1mg/ml
- 应用范围:
Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- 宿主:
Rabbit
- 适应物种:
Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
- 保质期:
一年
- 抗原来源:
Rabbit
- 目录编号:
TF4174R
- 级别:
I级
- 库存:
10
- 供应商:
晶风生物
- 标记物:
FITC/Alexa/CY357/BIo/HRP
- 克隆性:
Polyclonal
- 保存条件:
-20
- 形态:
Liquid
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived
- 规格:
50ul/100ul/200ul
产品名称:SIRT6沉默调节相关蛋白6抗体
产品规格:100ul/200ul(部分有50ul,如需更大包装或其他具体规格,请咨询客服)
研究领域:肿瘤 细胞生物 免疫学等
抗体来源:Rabbit
克隆类型:Polyclonal
交叉反应:Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,
产品应用:WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状:Liquid
浓 度:1mg/ml
免 疫 原:KLH conjugated synthetic peptide derived
亚 型:IgG
纯化方法:affinity purified by Protein A
储 存 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
SIRT6沉默调节相关蛋白6抗体相关抗体示例(非本抗体,如需本抗体,请联系客服索要说明书):
Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.
paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.
SIRT6沉默调节相关蛋白6抗体
Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Tissue/cell: rat colitis tissue; paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB staining
欢迎新老客户咨询订购:SIRT6沉默调节相关蛋白6抗体
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文献和实验7、9、10。它们的催化区域与酵母的 HDAl 蛋白同源,特异性地与细胞分化有关。 (3)第三类:最近发现酵母中的沉默信息调节因子―2(silentinformationregulator―2,SIR―2)也是一种组蛋白去乙酰化酶,人细胞中的 SIR―2 同源物也被鉴定出来。因此,SIR―2 及其相关蛋白构成了高等真核生物的第三类组蛋白去乙酰化酶。人类有 7 种 SIR―2 同源物,分别为 SIRT―1~7。 研究表明,酵母组蛋白去乙酰化酶 HDAl 及 RPD3 突变可使组蛋白H4普遍高乙酰化。HDAC
,并且 KIF5A 通过与 GABARAP、GABARAPL1 和 GABARAPL2 的相互作用而成为自噬通量的重要调节剂,因此假设 KIF5A 可能是参与自噬通量调节的关键因素。 细胞水平上:KIF5A 过表达明显恢复了 8μM TMT 处理的 Neuro-2a 细胞的细胞活力。PRM 靶向蛋白分析技术显示 Kif5a 过表达可恢复 tmt 处理的 neuro2a 细胞中自噬相关蛋白的水平。KIF5A 过表达可有效挽救 TMT 诱导的轴突运输缺陷。 动物水平上:腺相关病毒 (AAV)-KIF
在TATA box上开始,并随后招集其他相关蛋白进行装配。在装配的过程中,RNA聚合酶II也会结合其中而开始转录,也可能会受增强子或沉默子等元件的影响,结合上其他蛋白从而调控整个转录过程。 哺乳动物转录起始GTFs的装配 1.3 非结构性GTFs的功能 在基础转录装置中,大部分装配的因子只被证实具有结构性的角色。但是,其中仍有部分因子已被证实具有酶的功能,作用于转录的起始,如TFIIE。 值得注意的是,对于不同的启动子,GTFs的装配以及转录
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