- ￥1880
- 晶风生物
- TF1031R
- 中国
- 2025年06月28日
- Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- Rabbit
- Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
干扰素诱导跨膜蛋白1抗体
- 抗体英文名：
IFITM1
- 靶点：
细胞浆 细胞膜 分泌型蛋白
- 浓度：
1mg/ml
- 应用范围：
Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- 宿主：
Rabbit
- 适应物种：
Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
- 保质期：
一年
- 抗原来源：
Rabbit
- 目录编号：
TF1031R
- 级别：
I级
- 库存：
10
- 供应商：
晶风生物
- 标记物：
FITC/Alexa/CY357/BIo/HRP
- 克隆性：
Polyclonal
- 保存条件：
-20
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
KLH conjugated synthetic peptide derived
- 规格：
50ul/100ul/200ul
产品规格：100ul/200ul(部分有50ul，如需更大包装或其他具体规格，请咨询客服)
研究领域：肿瘤 细胞生物 免疫学等
抗体来源：Rabbit
克隆类型：Polyclonal
交叉反应：Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,
产品应用：WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状：Liquid
浓 度：1mg/ml
免 疫 原：KLH conjugated synthetic peptide derived
亚 型：IgG
纯化方法：affinity purified by Protein A
储 存 液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
IFITM1干扰素诱导跨膜蛋白1抗体相关抗体示例（非本抗体，如需本抗体，请联系客服索要说明书）：
Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.
paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.
IFITM1干扰素诱导跨膜蛋白1抗体
Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Tissue/cell: rat colitis tissue; paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB staining
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文献和实验Cancer Cell：中山大学刘卓炜团队揭示膀胱癌成纤维细胞新亚群介导肿瘤干性和化疗抵抗
。 既往研究表明成纤维细胞亚群的分化往往需要依赖某类细胞因子和转录因子的调控 [3]。研究者通过对单细胞测序数据的进一步分析发现 SLC14A1+成纤维细胞高表达干扰素响应通路的相关基因。随后通过体、内外实验证实肿瘤 cGAS-STING 来源的一型干扰素信号可以诱导这一亚群的分化并维持其功能，而 STAT1 则是调控这一成纤维亚群的关键转录因子。 图 2：研究机制示意图 该研究进一步阐明了恶性肿瘤成纤维细胞亚群的多样性及其功能的差异性，论证了干扰素信号在促进免疫活化和介导肿瘤进展的两面性，对于开发
学标志。有报道大约7%的慢性丙型肝炎患者也可检测到LKM-1抗体。LKM-3抗体见于10%-15%的慢性丁型肝炎患者。大约10%的自身免疫性肝炎既有抗LKM-1抗体，也有LKM-3抗体。但在自身免疫性肝炎中该抗体滴度要比病毒性肝炎中高的多。LKM-2只出现于由替尼酸引起的药物诱导性肝炎当中，因此LKM-1必须与LKM-2相区别开来。 临床意义： 抗LKM-1抗体为AIH- II型血清特异性抗体，敏感性为90%，在AIH中检出率较低(约
和数量充足，干细胞/原代细胞上清可在提取前采用超滤管（100KD）浓缩 3~5 倍后再提取。 3、使用EPF柱时堵塞如何处理？ 答：过滤柱的孔径在 220nm 左右。如果出现堵塞情况，可以将柱子旋转 180 度再次离心。若上室还有残留液体，需要使用新的 EPF 柱，该柱子可以单独购买（货号 UR90102）。 4、沉淀法的具体原理是什么？ 答：将高亲水性聚合物（聚乙二醇等）添加到含外泌体的溶液中后，外泌体周围的水分子被聚合物束缚，降低了外泌体的溶解度并诱导其随后的沉淀，使外泌体在低速离心下可以很容易
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