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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
末端补体复合物抗体
- 抗体英文名:
C5b-9
- 靶点:
细胞浆 细胞膜 分泌型蛋白
- 浓度:
1mg/ml
- 应用范围:
Elisa=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC=1:100-500,
- 宿主:
Rabbit
- 适应物种:
Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,Guinea Pig,
- 保质期:
一年
- 抗原来源:
Rabbit
- 目录编号:
TF2673R
- 级别:
I级
- 库存:
10
- 供应商:
晶风生物
- 标记物:
FITC/Alexa/CY357/BIo/HRP
- 克隆性:
Polyclonal
- 保存条件:
-20
- 形态:
Liquid
- 亚型:
IgG
- 免疫原:
KLH conjugated synthetic peptide derived
- 规格:
50ul/100ul/200ul
产品名称:C5b-9末端补体复合物抗体
产品规格:100ul/200ul(部分有50ul,如需更大包装或其他具体规格,请咨询客服)
研究领域:肿瘤 细胞生物 免疫学等
抗体来源:Rabbit
克隆类型:Polyclonal
交叉反应:Human,Mouse,Rat,Dog,Pig,Cow,Rabbit,Sheep,
产品应用:WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
性 状:Liquid
浓 度:1mg/ml
免 疫 原:KLH conjugated synthetic peptide derived
亚 型:IgG
纯化方法:affinity purified by Protein A
储 存 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
C5b-9末端补体复合物抗体相关抗体示例(非本抗体,如需本抗体,请联系客服索要说明书):
Sample:
Liver (Mouse) Lysate at 40 ug
Spleen (Mouse) Lysate at 40 ug
NIH/3T3 (Mouse) CellLysate at 30 ug
RAW246.7 (Mouse) CellLysate at 30 ug
Primary: Anti- IL12 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 35/36 kD
paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructions and DAB staining.
paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.
C5b-9末端补体复合物抗体
Blank control (blue line): Mouse spleen (blue).
Primary Antibody (green line): Rabbit Anti- IL12 antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Tissue/cell: rat colitis tissue; paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,at 37℃ for 20 min;
Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB staining
欢迎新老客户咨询订购:C5b-9末端补体复合物抗体
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文献和实验8 色多重荧光免疫组化方法揭示补体在早期类风湿关节炎发展中的作用
类风湿关节炎(RA)是一种以滑膜增生和炎症为特征的自身免疫性疾病。在血清阳性 RA 中发现自身抗体表明,由于关节中局部存在免疫复合物,补体系统激活可能发挥病理生理作用。尽管在开发类风湿关节炎新疗法方面取得了巨大进展,但目前尚无基于补体系统的疗法被批准用于临床治疗。 来自美国科罗拉多大学安舒茨医学院、英国威廉哈维研究所实验医学和风湿病学中心、伦敦玛丽女王大学伦敦医学和牙科学院和卡迪夫大学医学院的研究人员,为了探索特定补体因子在早期类风湿关节炎中的作用机制及其临床应用可能性,对于PEAC(早期
面,抗体的结合可以增强免疫细胞对抗原的识别和吞噬能力,进而增加免疫应答。另一方面,抗体的结合可以通过与免疫细胞表面的调节受体结合,抑制或增强免疫应答反应,帮助免疫系统在抗感染,抗肿瘤等过程中取得平衡的应答反应。 4、 抗原抗体复合物通过经典途径激活补体系统 补体系统是一种能够引起炎症和细胞溶解的蛋白质系统,IgG1、IgG2、IgG3和IgM类抗体与抗原结合形成免疫复合物激活补体系统,从而发挥补体介导的杀菌、溶菌作用。清除细菌、病毒等病原体。这主要在抗细菌和抗寄生虫感染中起作用。 5、介导细胞
Ⅲ型超敏反应的发病机制 Ⅲ型超敏反应或免疫复合物型超敏反应,是抗原抗体复合物沉积于组织内,通过激活补体而引起的细胞组织损伤。免疫复合物沉积的部位一方面取决于抗原在组织中的分布,同时也与循环免疫复合物在某些部位容易滞留有关。大的复合物可被肝、脾和骨髓中M甲捕获并清除,小的复合物存在于血循环中,但不会沉积,只有大小合适的复合物才能在局部滞留并活化补体系统,主要好发生在血管、肾赃、肺部、皮肤和关节等处。 组织局部免疫复合物沉积引起Ⅲ
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