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- 2025年12月25日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海逍鹏生物科技有限公司
- 库存：
充足
- 英文名：
DMEM/F12 1:1 mixture, with L-Glutamine and HEPES，SLE Grade（Endotoxin≤0.01EU/ml）
- 规格：
500ml
Dulbecco's Modified Eagle Medium /Nutrient Mixture F12 Ham（DMEM/F12 1:1 mixture） with L-Glutamine and HEPES was originally formulated for rat neuroblastoma cells and MDCK cells. The mixture is extremely nutritious and supports growth of a wide variety of cells including certain epithelial, endothelial and granulosa cells.
It is super low endotoxin SLETM grade，Endotoxin≤0.01EU/ml by LAL method. The production process and quality management system meet the requirements of cGMP and has passed ISO13485 certification.
It does not contain trace elements. Users are advised to review the literature for recommendations regarding medium supplementation and suitable cell lines.
Dulbecco's Modified Eagle Medium /Nutrient Mixture F12 Ham（DMEM/F12 1:1 mixture） with L-Glutamine and HEPES should be kept 2-8°C. The product is light -sensitive and therefore should not be left in the light. When stored in the dark under ideal conditions, the product is stable until the expiry date.
As with any other liquid media formulations, deterioration of liquid media may be recognized by any of the following characteristics, among others including:
(a). Color Change;
(b). Presence of clumping/flocculent debris/ granulation/ particulates\ precipitates or sediments;
(c). Insolubility;
(d). And/or decrease in expected performance parameters.
Any material described above should not be used and therefore discarded.
【Procedure】
1.Take a bottle from the defined storage conditions at 2-8°C and read the label.
2.Wipe the outside of the bottle with a disinfectant solution such as 70% ethanol.
3.Using aseptic/sterile technique under a laminar-flow culture hood, work according to established protocols.
4.Antibiotics may be added if desired.
【Quality Control】
Dulbecco's Modified Eagle Medium /Nutrient Mixture F12 Ham（DMEM/F12 1:1 mixture） with L-Glutamine and HEPES is tested for sterility, pH, osmolality and endotoxin concentrations. In addition, each batch is tested for cell growth using A549.
【Precaution and Disclaimer】
For research use only, not for clinical diagnosis and treatment.
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文献和实验IMDM 270～300DMEM/F-12 280～310F—10 270～300F—12 275～300培养基名称 渗透压范围（mOSM）L—15 280～320D-Hanks 280～300Hanks 280～300PBS 275～3005、细胞传代消化时所用胰酶浓度越高越好吗？ 不见得！因为胰蛋白酶溶液的消化能力是和溶液的pH、温度、胰酶浓度及溶液中是否含有Ca++， Mg++离子和血清等因素有关。通常情况下，pH 8.0， 温度37 ℃其作用能力最强。另外，钙、镁离子及血清均能大大降低其消化
条件。在MEM中培养的细胞，很可能在DMEM或M199中同样很容易生长。总之，首选MEM做粘附细胞培养、RPMI-1640做悬浮细胞培养，各种目的无血清培养的首选是AIM V培养基（SFM）。在开始进行新的细胞培养时，可以参考下表所列的条件： 7、为什么要热灭活血清？加热可以灭活补体系统。激活的补体参与溶解细胞事件，刺激平滑肌收缩，细胞和血小板释放组胺，激活淋巴细胞和巨噬细胞。在进行免疫学研究、培养ES细胞、昆虫细胞和平滑肌细胞时，推荐使用热灭活血清。 8、L－谷氨酰胺
, 10% 胎牛血清A549 上皮细胞 人 肺癌 F-12K, 10%胎牛血清A9 成纤维细胞 小鼠 结缔组织 DMEM, 10%胎牛血清AtT-20 上皮细胞 小鼠 垂体肿瘤 F-10, 15% 马血清和2.5% 胎牛血清BALB/3T3 成纤维细胞 小鼠 胚胎 DMEM, 10% 胎牛血清BHK-21 成纤维细胞 仓鼠 肾 GMEM, 10% 胎牛血清 或MEM
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