< 1.0 EU per μg of the protein as determined by the LAL method
生物活性
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells.When cells are added to POSTN-His coated plates (20μg/mL, 100μL/well), approximately >45% will adhere specifically after 30 minutes at 37℃.
蛋白构建
A DNA sequence encoding the human POSTN isoform 3 (NP_001129407.1) corresponding to amino acid (Met 1-Gln 781) was expressed with a C-terminal polyhistidine tag.
The recombinant human POSTN consists of 771 amino acids and has a predicted molecular mass of 86.4 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 66-80 kDa due to glycosylation.
缓冲液
Lyophilized from sterile PBS, 5% Trehalose, pH 7.0
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
参考文献
Kudo,Y. et al., 2007, Histol Histopathol. 22 (10):1167-1174.
Li, J.S. et al., 2007, World J Gastroenterol. 13 (39): 5261-5266.
Oku, E. et al., 2008, Int J Hematol. 88 (1): 57-63.
Hamilton, D.W. et al., 2008, J Cell Commun Signal. 2(1-2):9-17.
Puglisi, F.J et al., 2008, Clin Pathol. 61 (4): 494-498.
Conway, S. J. et al., 2008, Curr Genomics. 9 (8): 548-555.