Organism: homo sapiensTissue, cell type: kidney tissue (female), podocytes, visceral epithelial cells
Morphology: typical podocyte morphology, cytoplasmic extensions partially with arborized appearance
Life span extension: ectopic expression of hTERT
Quality: free from contaminations (bacteria incl. mycoplasma, fungi, HIV, HAV, HBV, HCV, Parvo-B19) and cross-contaminations
Morphology and growth
PODO/TERT256 cells are characterized by the typical, podocyte morphology.The cells can be grown continuously for at least 80 PDs without showing signs of growth retardation.
Expression of marker proteins
PODO/TERT256 cells express typical podocyte marker proteins such as Nephrin, WT-1 and Synaptopodin. Staining of actin fibers further underlines the typical podocyte morphology. Cell nuclei are counterstained with DAPI.
PAN induced toxicity – 2D
Undifferentiated and differentiated PODO/TERT256 cells grown in multi-well plates were treated with puromycin aminonucleoside. Using viability assay we could demonstrate that the cells loose viability and that this PAN induced toxicity could in part be rescued by addition of Mizoribin (MRZ).
PAN induced toxicity / 3D co-culture
PODO/TERT256 cells were co-cultured with telomerised microvascular endothelial cells HDMVEC/TERT164 in transwells. The thereby formed barrier retains bovine serum albumin (BSA), but can be disrupted by PAN. Mizoribin (MRZ) can rescue PAN induced injury.