BKS DB Mouse Mammary Microvascular Endothelial Cells
CAS号:
详询
供应商:
北京百奥创新科技有限公司
规格:
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Product Name
BKS DB Mouse Mammary Microvascular Endothelial Cells
Product Code
BKS DB MMMEC
Cat.No
ABC-TC5487
Species
BKS DB Mouse
Size/Quantity
1 vial
Shipping Info
Dry Ice
Storage
Liquid Nitrogen
Description
BKS db Mouse Mammary Microvascular Endothelial Cells are isolated from the mammary microvascular of Mice homozygous for the diabetes spontaneous mutation (Lepr/db) manifest morbid obesity, chronic hyperglycemia, pancreatic beta cell atrophy and become hypoRIemic. BKS db Mouse Mammary Microvascular Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in AcceGen’s Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vials. Each vial contains at least 0.5×106 cells per ml. The method we use to isolate primary endothelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with PECAM-1 antibody, following the application of magnetic beads pre-coated with secondary antibody. BKS db小鼠乳腺微血管内皮细胞分离自糖尿病自发突变纯合子小鼠乳腺微血管内皮细胞(Lepr/db),表现为病态肥胖、慢性高血糖、胰腺β细胞萎缩和低血凝。BKS db小鼠乳腺微血管内皮细胞在涂有明胶溶液的T25组织培养瓶中培养2分钟,然后在AcceGen的完全生长培养基中培养3-7天。文化被扩展。在运输之前,第3代细胞从烧瓶中分离出来,并立即在小瓶中冷冻保存。每瓶至少含有0.5×106个细胞每毫升。我们用于分离初级内皮细胞的方法是基于现有方法和我们专有的方法的结合开发的。在应用了涂有二抗的磁珠后,这些细胞被PECAM-1抗体预包覆。