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- Biotium
- 美国
- 00040-T
- 2025年07月07日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20°C
- 保质期：
1年
- 英文名：
phalloidin, CF555 00040-T
- 库存：
99
- 供应商：
Biotium
- CAS号：
1
A toxin isolated from the deadly Amanita phalloides mushroom. It is a bicyclic peptide that binds specifically to F-actin. When conjugated to a fluorescent label, it will stain actin filaments in fixed and permeabilized cells.
- Choice of 18 bright & photostable CF® dyes, biotin, or other common fluorophores
- Dye options for super-resolution and 2-photon imaging
- Excellent signal to background with negligible non-specific staining
Fluorescently-labeled phalloidins bind to F-actin with nanomolar affinity. Labeled phalloidins have similar affinity for both large and small filaments, binding in a stoichiometric ratio of about one phalloidin molecule per actin subunit in muscle and nonmuscle cells from various species of plants, animals and fungi. Different from antibodies, the binding affinity of phalloidin does not change significantly with actin among different species.
Fluorescently-labeled phalloidin is typically used to stain fixed and permeabilized cells. Labeled phalloidins are not cell-permeant and have therefore has not been used extensively with living cells, however they can be loaded into live cells via cationic liposomes.
Note: Phalloidin stock solutions are very stable. But binding of fluorescent phalloidins to cells is more labile than other probes, such as antibodies, and staining can become non-specific or lost over time. For best results, store phalloidin-stained samples in a suitable mounting medium at 4°C, protected from light. For certain phalloidin conjugates, especially CF®405M, CF®555, CF®647, and CF®680, we recommend imaging immediately or shortly after staining. Staining with our other CF® dye phalloidins usually is stable for up to a week when specimens are stored at 4°C, protected from light.
Superior CF® Dyes
Biotium’s next-generation CF® dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.
Super-Resolution Microscopy
CF®647 and CF®680 phalloidins are recommended mainly for STORM applications. Certain CF® dyes are compatible with various super resolution imaging techniques. The superior brightness, photostability, and photochemical switching properties of certain CF® dyes are ideal for 3-D SIM, 3-D STORM, and other super-resolution and single-molecule imaging approaches. Learn more about CF® dyes for super-resolution microscopy.
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文献和实验Development of Conditionally Immortalized Epithelial Cell Lines from CF and Non-CF Mice
Mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene are responsible for cystic fibrosis (CF) (1 ) and the primary defect in CF is the loss of cAMP-regulated anion conductance in the apical plasma membrane
Labeling Tubulin and Quantifying Labeling Stoichiometry
to tubulin. We have used it successfully to derivatize tubulin with succinimidyl esters of biotin, digoxigenin, and a wide range of fluorochromes such as tetramethylrhodamine, X-rhodamine, fluorescein, Oregon Green, Cy3, Cy5 and C2CF (bis-caged
3 分线外挣扎的你，看别人如何登顶 Nature、Science
能力。全剧终。统计分析又双叒叕来到了这个环节，但笔者惊讶的发现，只有三句话。数据用均数±SEM 表示，非配对 t 检验，P 后记其实早在 2008 年，Michael Welsh 领导的实验室就在 science 上发表了 CF 模式猪，解决了老鼠造模不典型的问题。正因为疾病模型的出现，为以后的研究打下了良好的基础。其后更在 2016 science 中回答了老鼠造模失败的原因。除了自建模型，活体杀菌能力测量的脑洞也是该文的亮点之一。笔者目前主要进行临床研究学习，作为基础研究的门外汉，窥觑这国自
