Triplex-Directed Site-Specific Genome Modification
The ability to target and manipulate specific mammalian genes has been a long sought goal in biotechnology and biomedicine. The realization of this goal has only recently become feasible, with advances in genetic engineering. Through the use of gene targeting strategies, it is possible to re ...
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High-Throughput Methods for SNP Genotyping
Single nucleotide polymorphisms (SNPs) are ideal markers for identifying genes associated with complex diseases for two main reasons. Firstly, SNPs are densely located on the human genome at about one SNP per approximately 500–1,000 base pairs. Secondly, a large number of commercial pla ...
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Use of DNA Combing to Study DNA Replicationin Xenopus and Human Cell-Free Systems
The Xenopus egg extract has become the gold standard for in vitro studies of metazoan DNA replication. We have used this system to study the mechanisms that ensure rapid and complete DNA replication despite random initiation during Xenopus early development. To this end we adapted the DNA combi ...
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Intracellular Ca2+ Imaging in C. elegans
Optical methods provide a noninvasive way to monitor calcium transients in Caenorhabditis elegans. Imaging techniques are particularly appealing in C. elegans because worms are optically transparent and can be imaged while fully intact. Furthermore, a variety of genetically enc ...
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