σ-Like plastid transcription factors
Work with chloroplast RNA polymerase from various plant species has established the presence of transcription factors that either act on the DNA template or on other polypeptides of the transcription complex. The first example was a 26-kDa polypeptide from maize chloroplasts, referr ...
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Staden: Searching for Restriction Sites
The program NIP contains a routine for finding and displaying the positions of the cut sites of restriction enzyme recognition sequences. Linear or circular sequences can be searched, and the results can be listed in various forms or displayed graphically. The recognition sequences to be se ...
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Bacteriophage -Based Expression Vectors
Bacteriophage λ has been in use as a cloning vector for over 20 years, and has been extensively used as an expression vector. A historical overview of λ as a cloning vector can be found in Murray (1), and a more recent review of λ vectors is in Chauthaiwale et al. (2). In general, λ is more useful as a tool for expressing foreign ...
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Transduction of Murine Hematopoietic Stem Cells and In Vivo Selection of Gene-Modified Cells
Hematopoietic stem cells (HSCs) were among the first targets of genetic manipulation for the purpose of treating human diseases. The translational aspects of the first human clinical trials were based on results obtained using the mouse as an experimental model. Murine studies have shown . ...
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