使用Foxp3鉴定Treg 用Anti-Mouse CD4 FITC、Anti-Mouse CD25 APC和Anti-Mouse/Rat Foxp3 PE 标记BALB/c小鼠 脾细胞. 右上象限是Tregs 细胞:CD4+Foxp3+ cells (左) and CD25+Foxp3+ cells (右).
在全血中检测Foxp3 eBioscience has simplified the analysis of Foxp3 in human whole blood. With the Foxp3 Whole Blood Staining Kit, it's now possible to analyze Foxp3 expression without isolating PBMCs from whole blood. The Foxp3 Whole Blood Staining Kit includes the PE-conjugated PCH101 clone for human Foxp3 and staining buffers specifically designed for fixation and permeabilization of cells in whole blood, resulting in robust detection of human Foxp3 expression.
参考文献 Mouse Foxp3 Biller, BJ., et al. 2007. Use of FoxP3 expression to identify regulatory T cells in healthy dogs and dogs with cancer. Vet Immunol Immunopathol. 116(1-2):69-78. [Intracellular staining for flow cytometry using FJK-16s in canine] Sakaguchi, S. et al. 2005. Treatment of advanced tumors with agonistic anti-GITR mAb and its effects on tumor-infiltrating Foxp3+CD25+CD4+ regulatory T cells. J. Exp. Med. 202: 885-891. [Intracellular staining for flow cytometry using FJK-16s] Fields, ML., et al. 2005. CD4+CD25+Regulatory T Cells Inhibit the Maturation but Not the Initiation of an Autoantibody Response. J. Immunol. 175: 4255 - 4264. [Intracellular staining for flow cytometry using FJK-16s]