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FUNGI - UNIVERSAL | |||||
Name | Region | Fragment Size | Sequence (5' to 3') | Reference | |
ITS1 | 18S | 570–590 | TCCGTAGGTGAACCTGCGG | White et al. 1990 | |
ITS4 | 28S | TCCTCCGCTTATTGATATGC | |||
ITS5 | 18S | 315 | GGAAGTAAAAGTCGTAACAAGG | White et al. 1990 | |
ITS2 | 5.8S | GCTGCGTTCTTCATCGATGC | |||
NS1 | 18S | 1100 | GTAGTCATATGCTTGTCTC | White et al. 1990 | |
NS4 | 18S | CTTCCGTCAATTCCTTTAAG | |||
NS1 | 18S | 1400 | GTAGTCATATGCTTGTCTC | White et al. 1990 | |
NS6 | 18S | GCATCACAGACCTGTTATTGCCTC | |||
NS5 | 18S | 700 | AACTTAAAGGAATTGACGGAAG | White et al. 1990 | |
NS8 | 18S | TCCGCAGGTTCACCTACGGA | |||
NS5 | 18S | 310 | AACTTAAAGGAATTGACGGAAG | White et al. 1990 | |
NS6 | 18S | GCATCACAGACCTGTTATTGCCTC | |||
White T. J., T. Bruns, S. Lee and J. Taylor, in PCR Protocols: A Guide To Methods And Applications, ed. M. A. Innis, D. H. Gelfand, J. J. Sninsky, T. J. White, Academic Press, San Diego, 1990, pp. 315–322. |
zjubell wrote:
FUNGI - UNIVERSAL Name Region Fragment Size Sequence (5' to 3') Reference ITS1 18S 570–590 TCCGTAGGTGAACCTGCGG White et al. 1990 ITS4 28S TCCTCCGCTTATTGATATGC ITS5 18S 315 GGAAGTAAAAGTCGTAACAAGG White et al. 1990 ITS2 5.8S GCTGCGTTCTTCATCGATGC NS1 18S 1100 GTAGTCATATGCTTGTCTC White et al. 1990 NS4 18S CTTCCGTCAATTCCTTTAAG NS1 18S 1400 GTAGTCATATGCTTGTCTC White et al. 1990 NS6 18S GCATCACAGACCTGTTATTGCCTC NS5 18S 700 AACTTAAAGGAATTGACGGAAG White et al. 1990 NS8 18S TCCGCAGGTTCACCTACGGA NS5 18S 310 AACTTAAAGGAATTGACGGAAG White et al. 1990 NS6 18S GCATCACAGACCTGTTATTGCCTC White T. J., T. Bruns, S. Lee and J. Taylor, in PCR Protocols: A Guide To Methods And Applications, ed. M. A. Innis, D. H. Gelfand, J. J. Sninsky, T. J. White, Academic Press, San Diego, 1990, pp. 315–322.
BACTERIA - UNIVERSAL | ||||
Primers | Sequence (5' to 3') | bp | Reference | |
63F | 16S | CAG GCC TAA CAC ATG CAA GTC | 1300 | Marchesi et al. 1998 |
1387R | GGG CGG WGT GTA CAA GGC | Marchesi et al. 1998 | ||
778R | AGG GTA TCT AAT CCT GTT TGC | Rosch & Bothe 2005 | ||
27F | 16S | AGA GTT TGA TCM TGG CTC AG | Gurtler & Stanisich 1996 | |
1492R | 16S | TAC GGH TAC CTT GTT ACG ACT T | ||
338F | Bacteria V3 Region (338-358) | *AC TCC TAC GGG AGG CAG CAG | Lane 1991 | |
518R | Universal V3 region (534-518) | ATT ACC GCG GCT GCT GG | Muyzer et al. 1993 | |
968F | Bacteria V6 region (968-983) | *AA CGC GAA GAA CCT TAC | Nübel et al. 1996 | |
1406R | Bacteria V9 region (1406-1392) | ACG GGC GGT GTG TAC | Lane et al. 1988 | |
72F | 16S | TGC GGC TGG ATC TCC TT | ||
38R | 16S | CCG GGT TTC CCCATT CGG | ||
*GC clamp added to the 5' end of the primer, 5'CGC CCG CCG CGC GCG GCG GGC GGG GCG GGG GCA CGG GGG G 3' |
zjubell wrote:
你搜索一下看,这篇文献是非常经典的。呵呵。同样,再给出一个细菌的。
BACTERIA - UNIVERSAL Primers Sequence (5' to 3') bp Reference 63F 16S CAG GCC TAA CAC ATG CAA GTC 1300 Marchesi et al. 1998 1387R GGG CGG WGT GTA CAA GGC Marchesi et al. 1998 778R AGG GTA TCT AAT CCT GTT TGC Rosch & Bothe 2005 27F 16S AGA GTT TGA TCM TGG CTC AG Gurtler & Stanisich 1996 1492R 16S TAC GGH TAC CTT GTT ACG ACT T 338F Bacteria V3 Region (338-358) *AC TCC TAC GGG AGG CAG CAG Lane 1991 518R Universal V3 region (534-518) ATT ACC GCG GCT GCT GG Muyzer et al. 1993 968F Bacteria V6 region (968-983) *AA CGC GAA GAA CCT TAC Nübel et al. 1996 1406R Bacteria V9 region (1406-1392) ACG GGC GGT GTG TAC Lane et al. 1988 72F 16S TGC GGC TGG ATC TCC TT 38R 16S CCG GGT TTC CCCATT CGG *GC clamp added to the 5' end of the primer, 5'CGC CCG CCG CGC GCG GCG GGC GGG GCG GGG GCA CGG GGG G 3'
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