运动训练和亚硒酸钠对雄性大鼠心脏组织中谷胱甘肽氧化酶和细胞色素c的影响

Introduction: Exercise training combined with supplements may be effective in enhancing antioxidant defense and mitochondrial function. Therefore, this study aimed to determine the response of glutathione peroxidase and cytochrome c to forced resistance swimming training and sodium selenite in the heart tissue of male rats.Methodology: Forty male Wistar rats at the age of 8 weeks were prepared and randomly divided into 4 groups:(1) resistance swimming training, (2) sodium selenite supplementation, (3) combined resistance swimming training and sodium selenite, and (4) control. Sodium selenite supplement was injected via gavage. Rats in the training group performed the forced resistance swimming training protocol. 48 h after the last session of the research protocol, rats were anesthetized with subcutaneous injections of ketamine and xylazine. Heart tissue was extracted and transported to the laboratory for further analysis. Using laboratory kits and the ELISA method, the response of glutathione peroxidase and cytochrome c to training and sodium selenite was examined. Mean, standard deviation, two-way analysis of variance, and Tukey's post hoc test were used to determine the differences between groups.Results: Glutathione peroxidase and cytochrome c showed significant increases in both the exercise, supplement, and exercise and supplement groups. Tukey's test showed that supplement consumption and forced resistance swimming training caused synergistic effects and increased the synthesis of the antioxidant glutathione peroxidase and cytochrome c (p ≤ 0.05).Discussion and conclusion: Exercise and sodium selenite consumption probably led to enhanced antioxidant capacity and improved mitochondrial response. In particular, the combination of these two interventions showed positive cellular adaptations, improved glutathione peroxidase metabolism, and increased selenoprotein synthesis.