更正:血管生成素-1受体Tie2区分牛尾椎髓核细胞的多能分化能力

Correction to: Angiopoietin-1 receptor Tie2 distinguishes multipotent differentiation capability in bovine coccygeal nucleus pulposus cells.

作者信息Adel Tekari, Samantha C W Chan, Daisuke Sakai, Sibylle Grad, Benjamin Gantenbein
PMID30704506
发布时间2019-01-31
DOI10.1186/s13287-019-1150-z

实验完整度

低

本文为更正声明,仅提供修正后的图2和材料方法片段,未包含完整实验过程。

摘要

Following publication of the original article in Stem Cell Research & Therapy [1], we would like to alert the reader that the immune-histological sections shown in Figure 2 bottom line are mistakenly the images from an experiment using a different Tie2+ antibody than originally reported in the manuscript (i.e. R&D, anti-human Tie2 labeled APC, cat.No:FAB3131A, clone:83715, mouse IgG) for the florescence associated cell sorting (FACS). This antibody has been previously tested in the group of Prof. Dr. Daisuke Sakai and was performed by Ms Tomoko Nakai, Tokai University. This antibody, however, was not found to be specific for bovine Tie2+ cells. The immune-histology procedure was correctly described using the PG antibody from Millipore. However, the pictures presented in Figure 2 in the last raw in the article of Tekari et al. [1] are not from the same experiment using the Tie2 antibody from Bioss, inc. clone bs-1300R, Bioss Antibodies, Woburn, MA, USA, as the publication reported.

实验结论

提炼研究问题、关键发现与证据,快速把握文章的核心贡献。

研究问题
文中未明确说明
核心机制
文中未明确说明
主要证据
文中未明确说明
研究意义
文中未明确说明

产品清单

实验环节名称品牌货号
抗人蛋白多糖抗体Milliporeclone
山羊抗小鼠抗体(Alexa Fluor 555)Invitrogen, Fisher-ScientificA-31621
Fluoroshield™封片剂abcamab104139
共聚焦激光扫描显微镜Carl ZeisscLSM710
Tie2一抗Biossbs-1300R
Alexa 488标记的二抗Molecular Probes, Life TechnologiesA-11008

关键环节

汇总复现实验时建议重点确认的条件及原文阅读提示。

环节核对要点
免疫组织化学
一抗(抗人蛋白多糖抗体,10 μg/ml,1:50稀释),封闭(10% FBS in PBS,1小时),二抗(羊抗鼠Alexa Fluor 555,1:200稀释),封片(Fluoroshield含DAPI),成像(共聚焦,10倍,4x4拼图)
阅读提示:原文第4页材料与方法部分
流式分选
Tie2一抗(Bioss, bs-1300R),二抗(Alexa 488标记,A-11008)
阅读提示:原文第4页材料与方法部分,或图2图注