利用FHF1A衍生肽FixR靶向心力衰竭心肌细胞中的致心律失常性晚期钠电流

Background: Enhanced late Na+ current (INa,L) in heart failure (HF) contributes to cardiomyocyte proarrhythmia. In addition to CaMKII (Ca2+/calmodulin-dependent protein kinase II), FGF (fibroblast growth factor) homologous factors 1-4 (FHF1-4) also modulate INa,L, and targeting these pathways may provide benefits in HF. Methods: Reverse-transcriptase quantitative polymerase chain reaction analysis of cardiac Na+ channel and FHF splice isoforms was performed in human arrhythmogenic HF with reduced ejection fraction (HFrEF) and in translational animal models of HFrEF and HF with preserved ejection fraction. We tested the effects of an FixR (FHF inhibiting x region) on cardiomyocyte INa,L and electrophysiology in rabbit HFrEF and murine models of HFrEF and HF with preserved ejection fraction. We also tested the in vivo electrophysiological effects of FixR via adenoviral delivery in transgenic mice with cardiomyocyte-specific overexpression of CaMKIIδC. Results: Expression of key FHF long isoforms (FHF1A in humans, FHF2S in rabbits, and FHF2VY in mice) that have inhibitory effects on INa,L was reduced in human, rabbit, and murine failing hearts. INa,L was markedly enhanced in both HFrEF and HF with preserved ejection fraction, and the FixR cell-penetrating peptide significantly reduced pathological INa,L in both forms of HF. FixR had no effect on transient peak Na+ current, L-type Ca2+ current, or major K+ currents in rabbit ventricular myocytes. FixR markedly attenuated proarrhythmogenic action potential changes (increased action potential duration, short-term variability, and alternans susceptibility) and delayed afterdepolarizations in both rabbit and murine failing cardiomyocytes. These cellular antiarrhythmic effects were mimicked by the selective INa,L inhibitor GS967 and the CaMKII inhibitor AIP (autocamtide-2 inhibitory peptide). FixR also attenuated QT prolongation and in vivo arrhythmia susceptibility in transgenic mice with cardiomyocyte-specific overexpression of CaMKIIδC. Conclusions: In HF, INa,L is increased, and FHF splice isoform expression is altered, allowing a novel mechanism to therapeutically target INa,L. FixR, an FHF1A-derived peptide, is a potent and selective INa,L inhibitor and has antiarrhythmic properties in both HFrEF and HF with preserved ejection fraction cardiomyocytes.