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Croquemort elicits activation of the immune deficiency pathway in ticks
摘要
The immune deficiency (IMD) pathway directs host defense in arthropods upon bacterial infection. In Pancrustacea, peptidoglycan recognition proteins sense microbial moieties and initiate nuclear factor-κB-driven immune responses. Proteins that elicit the IMD pathway in non-insect arthropods remain elusive. Here, we show that an Ixodes scapularis homolog of croquemort (Crq), a CD36-like protein, promotes activation of the tick IMD pathway. Crq exhibits plasma membrane localization and binds the lipid agonist 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol. Crq regulates the IMD and jun N-terminal kinase signaling cascades and limits the acquisition of the Lyme disease spirochete B. burgdorferi. Additionally, nymphs silenced for crq display impaired feeding and delayed molting to adulthood due to a deficiency in ecdysteroid synthesis. Collectively, we establish a distinct mechanism for arthropod immunity outside of insects and crustaceans.
实验方法
产品清单
| 名称 | 品牌 | 货号 |
|---|---|---|
| 溶菌肉汤（LB） | Millipore Sigma | -- |
| Barbour–Stoenner Kelly (BSK)-II 培养基 | -- | -- |
| L15C300 培养基 | -- | -- |
| 胎牛血清（FBS） | Millipore Sigma | -- |
| 胰蛋白胨磷酸盐肉汤 | BD | -- |
| 牛脂蛋白浓缩物 | MP Biomedicals | -- |
| 24孔板 | Corning | -- |
| 6孔板 | Millipore Sigma | -- |
| T25 培养瓶 | Greiner bio-one | -- |
| 小于扰RNA（siRNA） | Millipore Sigma | -- |
| Lipofectamine 3000 | ThermoFisher | -- |
| RIPA 裂解缓冲液 | Millipore Sigma | -- |
| Halt蛋白酶和磷酸酶抑制剂混合物 | Thermo Scientific | -- |
| Trizol | Ambion | -- |
| Silencer siRNA构建试剂盒 | Thermo Scientific | -- |
| 4D-Nucleofector 系统 | Lonza Bioscience | -- |
| SF 缓冲液 | Lonza Bioscience | -- |
| CellMask绿色质膜染色剂 | Invitrogen | -- |
| Hoechst核染色剂 | AAT Bioquest | -- |
| 荧光显微镜 | -- | -- |
| SpeedVac | Thermo Scientific | -- |
| EIA 缓冲液 | Cayman Chemical | -- |
| 20-羟基蜕皮酮EIA试剂盒 | Cayman Chemical | -- |