关于丁香通
公司信息
个人用户
企业机构
无忧采购 轻松科研
- 移动端
Lipid presentation by the protein C receptor links coagulation with autoimmunity
摘要
Antiphospholipid antibodies (aPLs) cause severe autoimmune disease characterized by vascular pathologies and pregnancy complications. Here, we identify endosomal lysobisphosphatidic acid (LBPA) presented by the CD1d-like endothelial protein C receptor (EPCR) as a pathogenic cell surface antigen recognized by aPLs for induction of thrombosis and endosomal inflammatory signaling. The engagement of aPLs with EPCR-LBPA expressed on innate immune cells sustains interferon- and toll-like receptor 7-dependent B1a cell expansion and autoantibody production. Specific pharmacological interruption of EPCR-LBPA signaling attenuates major aPL-elicited pathologies and the development of autoimmunity in a mouse model of systemic lupus erythematosus. Thus, aPLs recognize a single cell surface lipid-protein receptor complex to perpetuate a self-amplifying autoimmune signaling loop dependent on the cooperation with the innate immune complement and coagulation pathways.
实验方法
产品清单
| 名称 | 品牌 | 货号 |
|---|---|---|
| iCycler iQ热循环仪 | Bio-Rad | -- |
| GeneChip™ 小鼠基因2.0 ST芯片 | Affymetrix | -- |
| Affymetrix表达与转录组分析控制台软件 | Affymetrix | -- |
| FACS Canto流式细胞仪 | -- | -- |
| Acquity UPLC系统 | Waters | -- |
| Waters Acquity BEH色谱柱 | Waters | -- |
| Waters XEVO TQ-XS串联质谱仪 | Waters | -- |
| CD19微珠试剂盒 | -- | -- |
| 小鼠B1a细胞分离试剂盒 | -- | -- |
| α-APC微珠 | -- | -- |
| 小鼠或人pDC阴性选择试剂盒 | -- | -- |
| Zeiss LSM 710 NLO共聚焦激光扫描显微镜 | Zeiss | -- |
| Leica SP8共聚焦显微镜 | Leica | -- |
| Leica DFC9000 GTC sCMOS相机 | Leica | -- |
| FACS Canto I流式细胞仪 | -- | -- |
| Olympus BX51WI荧光显微镜 | Olympus | -- |
| 电荷耦合器件相机（ORCA-R2） | Hamamatsu Photonics | ORCA‐R2 |
| 实时成像系统eXcellence RT | Olympus | -- |
| FACSCalibur流式细胞仪 | Becton Dickinson | -- |
| 小鼠白蛋白ELISA试剂盒 | Bethyl Laboratories | -- |
| 肌酸测定试剂盒 | Creative BioMart | -- |