Arbor Assays 产品K031-H5 雌激素酮ELISA试剂盒说明书

雌激素酮ELISA试剂盒  Estrone ELISA Kit (5 Plate)说明书

 

产品名称：雌激素酮ELISA试剂盒  Estrone ELISA Kit (5 Plate)
产品货号：K031-H5

产品品牌：Arbor Assays
产品规格：5x96 well plate

产品链接：http://www.arborassays.com/documentation/inserts/K031-H.pdf
保存建议：The unopened kit must be stored at -20°C. Once opened the kit can be stored at 4°C up to the expirationdate on the kit label, except for the Estrone Conjugate. This must be stored at -20°C.
产品描述：雌激素酮ELISA试剂盒  Estrone ELISA Kit (5 Plate)
产品类型：竞争性ELISA
研究领域：生殖

背景介绍：BACKGROUND

Estrone, C18H22O2, also known as E1 or osterone (3-hydroxy-1,3,5(10)-estratrien-17-one) is a C-18 steroidhormone. Estrone is one of the three naturally occurring estrogens, the others being estradiol and estriol1.Estrone is produced primarily from androstenedione originating from the gonads or the adrenal cortex andfrom estradiol by 17-hydroxysteroid dehydrogenase2. Androstenedione is also converted into estrone byaromatase (CYP19) to estrone and is expressed in stromal and carcinoma or parenchymal componentsof breast cancer tissue3. Estrone concentrations in premenopausal mammals fluctuate according to themenstrual cycle. In premenopausal women, more than 50% of the estrone is secreted by the ovaries. Inprepubertal children, men and non-supplemented postmenopausal women the major portion of estroneis derived from peripheral tissue conversion of androstenedione. Interconversion of estrone and estradiolalso occurs in peripheral tissue. In humans, during the follicular phase of the menstrual cycle estrone levelsincrease slightly. The production of estrone then increases markedly to peak at around day 13. The peak isof short duration and by day 16 the estrone levels will be low. A second peak occurs at around day 21 of thecycle and if fertilization does not occur, then the production of estrone decreases.