Arbor Assays 产品K068-H5 孕酮代谢物ELISA试剂盒说明书

孕酮代谢物ELISA试剂盒 (5 Plate)，Progesterone Metabolites ELISA Kit (5 Plate)说明书

 

产品名称：孕酮代谢物ELISA试剂盒 (5 Plate)-Progesterone Metabolites ELISA Kit (5 Plate)
产品货号：K068-H5

产品品牌：Arbor Assays
产品规格：5x96wellplate

产品链接：https://www.arborassays.com/documentation/inserts/K068-H.pdf
保存建议：4˚C
产品描述：该孕酮代谢物ELISA试剂盒 (5 Plate)|Progesterone Metabolites ELISA Kit (5 Plate)
产品类型：竞争性ELISA
研究领域：生殖

检测原理：ASSAY PRINCIPLE

The DetectX® Progesterone Metabolites ELISA Kit is designed to quantitatively measure ProgesteroneMetabolites present in dried fecal extracts and urine. The Progesterone Metabolites ELISA Kit is acompetitive ELISA with a run time of 1.5 hours. Please read the complete kit insert before performingthis assay.A Progesterone Standard is provided to generate a standard curve for the assay and all samples shouldbe read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter platecoated with an antibody to capture rabbit antibodies. A Progesterone-peroxidase conjugate is added tothe standards and samples in the wells. The binding reaction is initiated by the addition of an antibody toProgesterone Metabolites to each well. As the Progesterone Metabolites concentration in the sampleincreases, the bound Progesterone-peroxidase conjugate decreases, resulting in a decrease in signaland vice versa.After an incubation, the plate is washed and substrate is added. The substrate reacts with the boundProgesterone-peroxidase conjugate. After a short incubation, the reaction is stopped, and the intensityof the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength.The concentration of the Progesterone Metabolites in the sample is calculated, after making suitablecorrection for dilution of the sample, using software available with most plate readers.