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小鼠肺上皮祖细胞系C22 (Clara)，ECACC #07021401
35 人阅读发布时间：2023-07-03 10:52
ECACC #07021401，C22 (Clara)，小鼠肺上皮祖细胞系C22 (Clara)，1 VIAL，准现货供应原装冻存管。
细胞链接：ECACC General Cell Collection: 07021401 C22 (Clara) (culturecollections.org.uk)
产品详细信息如下：
ECACC欧洲生物资源库提供经过验证和质量控制的细胞系、核酸和诱导多能干细胞 (iPSC)。并竭诚为研究界提供服务。
ECACC提供超过1100株细胞系，并可为部分细胞系可提供DNA、RNA等相关产品。
细胞链接：ECACC General Cell Collection: 07021401 C22 (Clara) (culturecollections.org.uk)
产品详细信息如下：
ECACC General Cell Collection: C22 (Clara)
| 提供单位 | European Collection of Authenticated Cell Cultures (ECACC) |
|---|---|
| 细胞类型 | Cell line |
| Collection: | ECACC General Collection |
| 货号 | 07021401 |
| 名称 | C22 (Clara) |
| Citation Guidance: | If use of this culture results in a scientific publication, it should be cited in the publication as: C22 (Clara) (ECACC 07021401) |
| Keywords: | Mouse lung epithelial progenitor cell小鼠肺上皮祖细胞系 |
| Cell Line Description: | A progenitor cell of the peripheral airway epithelium derived from transgenic H-2Kb-tsA58 mice capable of being passaged as an immortalised cloned cell line in culture. Cells will proliferate at 33°C in the presence of gamma-interferon, at 39°C in the absence of gamma-interferon cells will terminally differentiate. Supplied as frozen ampoules only. |
| 物种 | 小鼠 |
| 组织来源 | 肺 |
| CellType: | Epithelial |
| Growth Mode: | Adherent |
| Karyotype: | Not |
| GMO Status: | Genetically Modified Organism Class 1 (GMO1) |
| Biosafety Information: | Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK) All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises. Hyperlinks to MSDS documents: Frozen cell cultures Material Safety Data Sheet Growing cell cultures Material Safety Data Sheet Nucleic acids derived from cell cultures Material Safety Data Sheet |
| Subculture Routine: | Split confluent cultures 1:5 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using trypsin/EDTA;10% CO2; 33°C. Doubling time 48h |
| Culture Medium: | DMEM + 2mM Glutamine + 4% Foetal Bovine Serum (FBS) + 8 supplements: 0.25 μg/ml Endothelin-1
For guidance on the supplements and preparation of the culture medium please refer to the product document: T7 and C22(Clara) Culture Medium.pdf |
| Depositor: | Dr Daphne deMello, Department of Pathology, Phoenix Childrens Hospital, 1919 East Thomas Road, Phoenix, Arizona 85016, USA |
| Originator: | Yes |
| Country: | USA |
| References: | Demello et al., (2002) Generation and characterization of a conditionally immortalized lung Clara cell line from the H-2K In Vitro Cell. Dev. Biol.-Animal 38: 154-164 |
| Additional Bibliography: | Not Available |
| Patents: | None Specified By Depositor |
| Release Conditions: | No |
ECACC欧洲生物资源库提供经过验证和质量控制的细胞系、核酸和诱导多能干细胞 (iPSC)。并竭诚为研究界提供服务。
ECACC提供超过1100株细胞系，并可为部分细胞系可提供DNA、RNA等相关产品。
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