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DJ-1 (D29E5) XP® Rabbit mAb
synthetic peptide corresponding to residues surrounding Lys148 of human DJ-1 protein
W, IP, IF-IC
H,M,R,Hm,Mk
详见说明书
CST
大量
详见MSDS文件
多克隆
-20°c
40 ul (4 western blots)/100 ul (10 western blots)/carrier free & custom formulation / quantity
规格: | 产品价格: | 询价 | |
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规格: | 40 ul (4 western blots) | 产品价格: | 询价 |
规格: | 100 ul (10 western blots) | 产品价格: | 询价 |
规格: | carrier free & custom formulation / quantity | 产品价格: | 询价 |
pathwaymore infoapplication referencesdatasheet PDFMSDS PDFprotocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
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W IP IF-IC | H M R Hm Mk | Endogenous | 22 | Rabbit IgG |
Protocols | |
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Specificity / Sensitivity | DJ-1 (D29E5) XP® Rabbit mAb recognizes endogenous levels of total DJ-1 protein. |
Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys148 of human DJ-1 protein. Western BlottingWestern blot analysis of extracts from MEF wild-type, MEF DJ-1 (-/-), HeLa, and C6 cells using DJ-1 (D29E5) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). (MEF wild-type and MEF DJ-1 (-/-) cells were kindly provided by Dr. Philipp Kahle, University of Tübingen, Germany). IF-ICConfocal immunofluorescent analysis of MEF wild-type (left) or MEF DJ-1 (-/-) (right) cells using DJ-1 (D29E5) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). (MEF wild-type and MEF DJ-1 (-/-) cells were kindly provided by Dr. Philipp Kahle, University of Tübingen, Germany). |
Background | Parkinson's disease (PD) is characterized by the presence of Lewy bodies (intracellular inclusions) and by the loss of dopaminergic neurons. Research studies have shown that mutations in α-synuclein, Parkin, and DJ-1 are linked to PD (1). α-synuclein is a major component of the aggregates found in Lewy bodies. Parkin is involved in protein degradation through the ubiquitin-proteasome pathway, and investigators have shown that mutations in Parkin cause early onset of PD (1). Loss-of-function mutations in DJ-1 cause early onset of PD, but DJ-1 is associated with multiple functions: it cooperates with Ras to increase cell transformation, it positively regulates transcription of the androgen receptor, and it may function as an indicator of oxidative stress (2-5). Dopamine D2 receptor-mediated functions are greatly impaired in DJ-1 (-/-) mice, resulting in reduced long-term depression (6).
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Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know! |
Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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