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cav2.2抗体
- 参考价格： ¥3025.0 - 5225.0
- 品 牌： abgent
- 货 号： AG1460
- 适应物种： 人,小鼠,大鼠
- 应用范围： 免疫印迹,免疫组化,免疫细胞化学
- 抗体类型： 一抗
- 主要序列号： Q02294
- 预吸附控制： 1 µg peptide per 1 µg antibody.
产品概览
| 背景： | Voltage dependent Ca2+ channels (CaV channels) are pivotal players in many physiological roles such as secretion, contraction, migration and excitation.1The voltage dependent Ca2+ channels are composed of several subunits; α1, β, α2δ and γ. CaV channels were originally divided into six physiological types: L, N, P, Q, R, and T type.The CaV2.2 (formally named α1B) composes the α1 poreforming subunit for the N type Ca2+ channel family. They are involved in neurotransmitter release from central neurons, including glutamate, γ-aminobutyric acid, acetylcholine, dopamine and noradrenaline.2The CaV2.2 is expressed preferentially in the central nervous system, where along with CaV2.1, it is responsible for pre-synaptic Ca2+ influx and neurotransmitter release.1,3The CaV2.2 channel is negatively regulated by many different GPCRs. There are two ways that this is done: either by directly binding Gβγ to the channel or by an indirect mechanism involving second messenger and channel phosphorylation.4ω-Conotoxin GVIA (#C-300) is a specific blocker of Cav2.2 Ca2+ channels. It specifically blocks N-type Cav channels by binding to the Cav2.2 α1 subunit (α1B) and its action is only partially reversible.5,6REFERENCES1. Tsunemi, T. et al. (2002) J. Biol. Chem. 277, 7214.2. Beuckmann, C.T. et al. (2003) J. Neurosci. 23, 6793.3. Scheuber, A. et al. (2004) J. Neurosci. 24,10402.4. Dascal, N. (2001) Trends Endocrinol. Metab. 12, 391.5. McCleskey, E. W. et al. (1987) Proc. Natl. Acad.Sci. USA 84, 4327.6. Feng, Z. P. et al. (2001) J. Biol. Chem. 276, 15728. |
| 其他名字： | Alpha1B, Cacna1b |
靶点信息
| 基因id： | 257648 |
应用与保存
| 配制： | 25 µl, 50 µl or 0.2 ml deionized water, depending on the sample size. |
| 配制前保存条件： | Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C. |
| 配制前对照抗原保存条件： | Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C. |
| 配制后保存条件： | The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min). |
| 配制后对照抗原保存条件： | -20ºC. |
| 配制后抗体浓度： | 0.8 mg/ml. |
| 应用详情： | Western blot analysis (WB):- Rat primary submucosa cells (see Rehn, M. et al. (2013) in Product Citations).- Mouse synaptosome (1:300) (see Saggu et al. (2013) in Product Citations). Immunoprecipitation (IP):- Transfected tsA201 cells (1:200) (see Marangoudakis, S. et al. (2012) in Product Citations).Immunohistochemistry (IH):- Rat retinal sections (1:1000) (see Sargoy, A. et al. (2013) in Product Citations).- Rat intracardiac ganglia and stellate ganglia neurons (see Tu, H. et al. (2014) in Product Citations).- Rat retinal sections (1:1000-1:1500) (see Liu, X. et al. (2013) in Product Citations). Immunocytochemistry (IC):- Rat primary submucosa cells (1:100) (see Rehn, M. et al. (2013) in Product Citations).- NCI-H295R human adrenocortical cell line (H295R) (1:100) (see Aritomi, S. et al. (2011) in Product Citations). |
| 稀释浓度： | WB~~1:200IHC~~1:100ICC~~1:200 |
其他
| 参考文献： | 1. Tsunemi, T. et al. (2002) J. Biol. Chem. 277, 7214.2. Beuckmann, C.T. et al. (2003) J. Neurosci. 23, 6793.3. Scheuber, A. et al. (2004) J. Neurosci. 24,10402.4. Dascal, N. (2001) Trends Endocrinol. Metab. 12, 391.5. McCleskey, E. W. et al. (1987) Proc. Natl. Acad.Sci. USA 84, 4327.6. Feng, Z. P. et al. (2001) J. Biol. Chem. 276, 15728. |
| 对照实验相关产品： | Control peptide antigen (supplied with the antibody free of charge). |
| 对照抗原配制： | 100 µl water. |
| 标记物： | Affinity purified antibody, lyophilized powder |
| 分子量： | 262256 Da |
| 同源性： | Mouse - 15/17 amino acid residues identical; human 11/17 amino acid residues identical; rabbit - 11/17 amino acid residues identical. |
| 提取： | Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3. |
| 验证肽： | Confirmed by amino acid analysis and mass-spectrography. |
| 规格： | 0.2 ml | 价格： | ¥5225.00 |
| 规格： | 50 µl | 价格： | ¥4125.00 |
| 规格： | 25 µl | 价格： | ¥3025.00 |
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