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cav3.2抗体
- 参考价格： ¥4125.0 - 5225.0
- 品 牌： abgent
- 货 号： AG1352
- 适应物种： 人,小鼠,大鼠
- 应用范围： 免疫印迹,免疫组化,免疫细胞化学
- 抗体类型： 一抗
- 主要序列号： Q9EQ60
- 配制后抗体浓度： 0.8 mg/ml.
产品概览
| 背景： | T-type Ca2+channels play an important role in many cellular processes such as hormone secretion, neurotransmitter release and cell differentiation. T-type channels are also known to participate in the pacemaker activities of heart and neurons including thalamic neurons.1Three genes encoding T-type Ca2+channels have been cloned and designated as Cav3.2 (α1H), Cav3.1 (α1G) and Cav3.3 (α1I).1-3 The Cav3.2 (α1H) channel is widely expressed in various tissues, brain, heart, liver and testis.Involvement of Cav3.2 Ca2+channels in several pathologies has been described. Overexpression of the Cav3.2 channel was described in prostate cancer cells that are associated with more aggressiveness, invasiveness and poor prognosis.4Several point mutations discovered in the Cav3.2 channel that affect gating of the channel were found to be associated with Childhood Absence Epilepsy.5One year old mice, deficient in Cav3.2 channel, exhibited severe cardiac pathology, fibrosis, necrosis, lymphocyte infiltration and abnormal coronary function compared to wild-type mice.6 Recently, it has been demonstrated that T-type channels are expressed in DRG neurons and that small and medium-diameter primary afferent neurons in the dorsal horn expresses almost exclusively the Cav3.2 Ca2+channels. This might indicate a possible role for Cav3.2 in nociception.7 REFERENCESReferences 1. Park, J.Y.et al. (2004)J. Biol. Chem. 279, 21707. 2. Chemin, J.et al.(2001)Eur. J. Neurosci. 14, 1678. 3. Monteil, A.et al. (2000)J. Biol. Chem. 275, 16530. 4. Gackière, F. et al.(2008)J. Biol. Chem. 283, 10162. 5. Khosravani, H.et al. (2004)J. Biol. Chem. 279, 9681. 6. Chen, C.C.et al. (2003)Science302,1416. 7. Bourinet, E.et al.(2005)EMBO J. 24, 315. |
| 其他名字： | Cav3.2 |
靶点信息
| 基因id： | 114862 |
应用与保存
| 配制： | 50 µl or 0.2 ml deionized water, depending on the sample size. |
| 配制前保存条件： | Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C. |
| 配制前对照抗原保存条件： | Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C. |
| 配制后保存条件： | The reconstituted solution can be stored at 4ºC for up to 2 weeks. For longer periods, small aliquots should be stored at -20ºC or below. Avoid multiple freezing and thawing. The further dilutions should be made using a carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min). |
| 配制后对照抗原保存条件： | -20ºC. |
| 应用详情： | Western blot analysis (WB):- Mouse lung and brain lysate (1:200) (see Wan, J.et al.(2013) in Product Citations).- Human CaV3.2 transfected in HEK-293 cells (1:100) (see Markandeya, Y.S.et al.(2011) in Product Citations).Immunoprecipitation (IP):- Rat brain lysates (5 μg) (see Weiss, N.et al.(2012) in Product Citations). |
| 稀释浓度： | WB~~1:200IHC~~1:50ICC~~1:200 |
其他
| 参考文献： | References 1. Park, J.Y.et al. (2004)J. Biol. Chem. 279, 21707. 2. Chemin, J.et al.(2001)Eur. J. Neurosci. 14, 1678. 3. Monteil, A.et al. (2000)J. Biol. Chem. 275, 16530. 4. Gackière, F. et al.(2008)J. Biol. Chem. 283, 10162. 5. Khosravani, H.et al. (2004)J. Biol. Chem. 279, 9681. 6. Chen, C.C.et al. (2003)Science302,1416. 7. Bourinet, E.et al.(2005)EMBO J. 24, 315. |
| 对照实验相关产品： | Control peptide antigen (supplied with the antibody free of charge). |
| 对照抗原配制： | 100 µl water. |
| 标记物： | Affinity purified antibody, lyophilized powder |
| 分子量： | 261113 Da |
| 同源性： | Mouse, bovine and canis - 14/15 amino acid residues identical; human - 13/15 amino acid residues identical. |
| 提取： | Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3. |
| 预吸附控制： | 1 µg peptide per 1 µg antibody. |
| 验证肽： | Confirmed by amino acid analysis. |
| 规格： | 50 µl | 价格： | ¥4125.00 |
| 规格： | 0.2 ml | 价格： | ¥5225.00 |
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