Protein C4色谱柱对铁蛋白和脱铁铁蛋白的分离AN48
Introduction
While reversed phase chromatography (RPC) is often used for the analysis of small molecular weight compounds, there are also various standard applications for the separation of biomolecules, such as proteins.Conventional reversed phase HPLC packing materials with 8-14 nm pore sizes are not generally suitable for the analysis of large intact proteins because the analytes are not able to access the surface area within these pores. In answer to the need for an RPC column optimized for protein analysis, Tosoh Bioscience developed a wide pore 30 nm, silica-based butyl (C4) column, the TSKgel Protein C4-300.
The packing of the TSKgel Protein C4-300 column, prepared by polymeric binding of butyl (C4) alkyl groups, reduces the protein adsorption on the stationary phase compared to a C18 stationary phase. The stationary phase is fully endcapped with trimethylsilyl (TMS) groups to prevent interaction with free silanol groups. This incurs higher stability of the phase and reduction of peak tailing.
This application note discusses the analysis of large metalloproteins and monoclonal antibodies using a TSKgel Protein C4-300 column.