UltraSW Aggregate色谱柱SEC-MS法分离单抗多聚体的应用（AN68）
Introduction
The use of mass spectrometry as a means of detection is becoming increasingly more common among research laboratories in the field of proteomics. After more than 15 years of research, LC-MS systems are now more robust, and used more often for routine analyses which are nearly unachievable by any other mode of detection.
Separation of protein aggregates from their native species is often performed using Size Exclusion Chromatography (SEC), as this mode allows for the analysis of various components in a sample on the basis of their hydrodynamic radius in solution. Conventional SEC separations make use of relatively high ionic strength mobile phase compositions in an effort to minimize ionic interactions between the analyte and stationary phase. Due to the substantial amount of salt present in the mobile phase, on-line interfacing with mass spectrometry is not feasible due to the inevitable contamination of the MS ion source by the mobile phase salts. In order to make SEC-MS an applicable technique, volatile, MS-friendly mobile phase compositions must be implemented to avoid damage to the MS system. The challenge of using such mobile phase compositions exists due to the absence of salts which hinder ionic interactions during a separation.
This Application Note illustrates the effective use of MS-friendly mobile phase compositions in the analysis of monoclonal antibody aggregates using a TSKgel UltraSW Aggregate SEC column. The TSKgel UltraSW Aggregate column demonstrates high stability and low reactivity via ionic interactions even in low salt/no salt environments most likely due to the diol coating of the silica stationary phase.
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