Name |
Recombinant Rat HB-EGF (Recombinant Rat Heparin-binding EGF-like Growth Factor; rRtHB-EGF);重组大鼠肝素结合表皮生长因子样生长因子 |
Synonyms |
Dtr; Dts; Hegfl; diphtheria toxin receptor (heparin-binding epidermal growth factor-like growthfactor); DTRHEGFLdiphtheria toxin receptor (heparin-binding EGF-like growth factor); DTS; DTSF; heparin-binding EGF-like growth factor; heparin-binding epidermal growth factor; proheparin-binding EGF-like growth factor |
Purity |
>95% by SDS-PAGE and HPLC analyses. |
Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1ng/ml, corresponding to a specific activity of >1.0×106IU/mg. |
Physical Appearance |
Sterile Filtered lyophilized (freeze-dried) liquid-like powder. |
Formulation |
Lyophilized from a 0.2μm filtered concentrated solution in PBS, 300mM NaCl, pH7.4, 5% trehalose. |
Endotoxin |
Less than 1EU/μg of rRtHB-EGF as determined by LAL method. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions. |
Category |
Cytokine |
Background |
Heparin-binding epidermal growth factor (HB-EGF)-like growth factor (EGF) is found in cerebral neurons. Its expression is increased after hypoxic or ischemic injury, which also stimulates neurogenesis. HB-EGF has been implicated as a participant in a variety of normal physiological processes such as blastocyst implantation and wound healing, and in pathological processes such as tumor growth, SMC hyperplasia and atherosclerosis. HB-EGF is an 87 amino acid mitogenic and chemotactic glycoprotein containing an EGF-like domain with six conserved cysteine residues. In addition, it shares about 73% and 76%a.a. sequence identity with murine and rat HB-EGF. |
Amino Acid Sequence |
DLEGTDLDLF KVAFSSKPQA LATPGKEKNG KKKRKGKGLG KKRDPCLKKY KDYCIHGECR YLKELRIPSC HCLPGYHGQR CHGLTL |