来源 |
用途 |
交叉反应性 |
理论分子量 |
实际分子量 |
Rabbit |
WB |
M |
129/133kDa |
160kDa |
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ChIP, Chromatin Immunoprecipitation Assay; ChIP-seq, ChIP-sequencing. H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected. 配套提供了Western一抗稀释液,可以用于Western检测或其它适当用途时的一抗稀释。 建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):
WB |
IP |
IF |
IHC |
ICC |
FC |
ChIP |
ChIP-seq |
1:500-1:2000 |
- |
- |
- |
- |
- |
- |
- |
抗体详细信息如下:
About this Antibody |
Name |
EGF Rabbit Polyclonal Antibody |
Category |
Rabbit Polyclonal Antibody (pAb); Primary antibody |
Isotype |
IgG |
Purification method |
Affinity purification |
Positive samples |
Mouse kidney, 293T |
Cellular location |
Membrane, Single-pass type I membrane protein |
Customer validation |
- |
About the Immunogen |
Immunogen |
Recombinant protein of human EGF |
Sequence |
- |
Gene ID |
1950 |
Swiss Prot |
P01133 |
Synonyms |
EGF; HOMG4; URG; epidermal growth factor |
Category |
Jak/Stat:IL-32 Signaling; NF-κB Signaling; ErbB/HER Signaling |
Background |
Epidermal growth factor (EGF) is the founding member of the EGF family that also includes TGF-alpha, amphiregulin (AR), betacellulin (BTC), epiregulin (EPR), heparin binding EGF like growth factor (HB EGF), epigen, and the neuregulins (NRG)-1 through -6. Members of the EGF family share a structural motif, the EGF like domain, which is characterized by three intramolecular disulfide bonds that are formed by six similarly spaced conserved cysteine residues. All EGF family members are synthesized as type I transmembrane precursor proteins that may contain several EGF domains in the extracellular region. The mature proteins are released from the cell surface by regulated proteolysis. The 1207 amino acid (aa) human EGF precursor contains nine EGF domains and nine LDLR class B repeats. The mature protein consists of 53 aa and is generated by proteolytic excision of the EGF domain proximal to the transmembrane region. Mature human EGF shares 70% aa sequence identity with mature mouse and rat EGF. EGF is present in various body fluids, including blood, milk, urine, saliva, seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid. Four ErbB (HER) family receptor tyrosine kinases including EGFR/ErbB1, ErbB2, ErbB3 and ErbB4, mediate responses to EGF family members. These receptors undergo a complex pattern of ligand induced homo- or hetero-dimerization to transduce EGF family signals. EGF binds ErbB1 and depending on the context, induces the formation of homodimers or heterodimers containing ErbB2. Dimerization results in autophosphorylation of the receptor at specific tyrosine residues to create docking sites for a variety of signaling molecules. Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture. |
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