Antibody Pairs: |
|
|
Der p 1 |
10B9/5H8 |
|
Der f 1 |
6A8/4C1 |
|
Fel d 1 |
6F9/3E4 |
|
Can f 1 |
10D4/6E9 |
|
Bla g 2 |
1F3/4C3 |
Sensitivity: |
Dependent on the analyte: 0.02ng/ml – 0.06ng/mlg/ml |
Lower Limit of Detection: |
Dependent on the analyte: 0.02ng/ml – 0.06ng/ml |
Assay Performance: |
Intra-assay CV: <5% Inter-assay CV: <10% |
Contents: |
Multiplex reagents for 1 microtiter plate: Premixed 5-plex Antibody-coupled Fluorescent, Universal Allergen Standard, Premixed Biotinylated Detector mAb, Quality Control Samples High/Low, Streptavidin-Phycoerythrin, Microtiter Filter Plate. |
Storage: |
Store all kit components at 4°C. If not used within 7 days of receipt: Store the Universal Allergen Standard (ST-UAS) and Quality Control Samples (QC-MRA-H/L) at -20°C until used. DO NOT freeze microspheres (MS-P5B) and detector antibodies (BI-MRA-P5B). |
Not Provided: |
Phosphate Buffered Saline, pH 7.4, containing 0.02% Tween 20 (PBS-T), 0.22µm filtered Bovine Seruma Albumin |
Notes: |
|
Product Resources: |
MRA-P5B Certificate of Analysis |
Related Products: |
|
For Research Use Only: Not for Diagnostic or Therapeutic Use |
|
Certificate of Analysis |
Microspheres: |
Premixed 5-plex Antibody-coupled Fluorescent Microspheres |
|
Analyte |
Bead Region |
|
Der p 1 |
33 |
|
Der f 1 |
51 |
|
Fel d 1 |
58 |
|
Can f 1 |
20 |
|
Bla g 2 |
47 |
Product Code: |
MS-P5B |
Vial: |
Blue top, 75 µL |
Specificity: |
mAb against Der p 1, Der f 1, Fel d 1, Can f 1 and Bla g 2. |
|
|
Certificate of Analysis |
Allergen Standard: |
Universal Allergen Standard |
Product Code: |
ST-UAS |
Vial: |
Yellow top, 50 µL |
Composition: |
A formulation of eight purified natural allergens prepared in 1% BSA/50% glycerol/PBS, pH 7.4 |
Concentration/Calibration: |
|
Universal Allergen Standard
|
Protein Measurement
|
Concentration (ng/ml)
|
Der p 1
|
Amino-acid analysis
|
2500
|
Der f 1
|
Amino-acid analysis
|
2500
|
Der p 2
|
Amino-acid analysis
|
1000
|
Fel d 1
|
Amino-acid analysis
|
1000
|
Can f 1
|
Amino-acid analysis
|
2500
|
Rat n 1
|
Amino-acid analysis
|
1000
|
Mus m 1
|
Amino-acid analysis
|
250
|
Bla g 2
|
Amino-acid analysis
|
2500
|
|
|
Certificate of Analysis |
Detector Antibody: |
Premixed Biotinylated Detector mAb |
|
The following biotinylated mAb are premixed in the vial provided: |
|
Analyte |
mAb |
|
Der p 1 |
5H8 |
|
Der f 1 |
4C1 |
|
Fel d 1 |
3E4 |
|
Can f 1 |
6E9 |
|
Bla g 2 |
4C3 |
Product Code: |
BI-MRA-P5B |
Vial : |
Brown top, 100 µL |
Biotinylation: |
Biotinylated using EZ-Link Sulfo-NHS-LC Biotinylating Agent and titrated for use in the array. Prepared in 1% BSA/50% glycerol/PBS, 0.22µm filtered, preservative free. |
Quality Control Samples: |
High/Low |
Product Code: |
QC-MRA-H and QC-MRA-L |
Two vials: |
Purple top, 50 µL |
Composition: |
A formulation of high/low positive controls prepared in PBS-T 0.02%, 1% BSA, pH 7.4, (concentrations are lot-dependent) |
|
Fluorophore: |
Streptavidin-Phycoerythrin |
Product Code: |
SAP-MRA |
Vial: |
Pink top, 50 µL |
|
Microtiter Filter Plate |
|
Product Code: |
FP-MRA Millipore MultiScreen BV Millipore catalog # MABVN1250 |
References |
|
Earle CD, King EM, Tsay A, Pittman K, Saric B, Vailes L, Godbout R, Oliver KG, Chapman MD. High-throughput fluorescent multiplex array for indoor allergen exposure assessment. J Allergy Clin Immunol, 2007; 119:428-433. |
Wright GR, Howieson S, McSharry C, McMahon AD, Chaudhuri R, Thompson J, Donnelly I, Brooks RG, Lawson S, McAlpine L, King EM, Chapman MD, Wood S, Thomson NC. Effect of improved home ventilation on asthma control and house dust mite allergen levels. Allergy, 2009, in print. |
(For complete reference list see MRA-P5B Certificate of Analysis) |