Cleavage Sites Table for Fusion Proteins:
PROTEASE |
COMPANY |
Protease Capture |
Thrombin Leu-Val-Pro-Arg�Gly-Ser |
Amersham-Biosciences, Novagen, SIGMA, Roche |
Benzamidine-Agarose |
Factor Xa Ile-Glu/Asp-Gly-Arg� |
Amersham-Biosciences, New England Biolabs, Roche |
Benzamidine-Agarose |
Enterokinase Asp-Asp-Asp-Asp-Lys� |
New England Biolabs, Novagen, Roche |
Trypsin Inhibitor-Agarose |
TEV protease Glu-Asn-Leu-Tyr-Phe-Gln�Gly |
Invitrogen – Life Technologies |
Ni-NTA (6His recomb. TEV) |
PreScission Leu-Glu-Val-Leu-Phe-Gln�Gly-Pro |
Amersham-Biosciences |
GSTrap for GST fusion enzyme |
TAGZyme His-tag removal by Exoproteolytic Digestion |
Qiagen |
Ni-NTA (6His recomb. enzyme) |
Intein Site dithiothreitol cleavage |
New England Biolabs |
DTT elimination by dialysis |
HRV 3C Protease Leu-Glu-Val-Leu-Phe-Gln�Gly-Pro |
Novagen |
Ni-NTA (6His recomb. enzyme) |
SUMO Protease recognize the tertiary structure of the ubiquitin-like (UBL) protein, SUMO |
Invitrogen – Life Technologies, LifeSensors |
Ni-NTA (6His recomb. enzyme) |
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