Catecholaminergic Cell Lines for the Study of Dopamine Metabolism and Neurotoxicity
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The selective loss of melanin-containing dopaminergic neurons in the substantia nigra pars compacta and locus coeruleus has
motivated a large number of preclinical studies aimed at understanding the molecular mechanisms involved in the neurodegeneration
of dopaminergic neurons in Parkinson’s disease. To perform these preclinical studies with catecholaminergic neurotoxins such
as 6-hydroxydopamine, MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine), rotenon, paraquat, aminochrome, etc. requires to
select cell model that expresses dopaminergic features such as dopamine transport, formation of neuromelanin, release of dopamine,
and monoamine oxidase. The most used model cell lines are PC12 and SH-SY5Y cell lines due to their catecholaminergic properties,
but we review several other cell lines that can be useful.
