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        Fluorescence Microscopy Methods

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        The last decade has seen an extraordinary improvement in fluorescence microscopy instruments. These have been mainly in the development of epi-illumination systems, including dichroic beam splitters, matching excitation and barrier filters, and special objectives (see ref. 1 for background information). Routinely we use a JenaLumar fluorescence microscope (Zeiss, Jena, Germany) equipped with oil immersion wide field � 100 objective with an adjustable iris diaphragm. According to our experience, the best documentation is obtained when the photographs are taken on Kodak Tri X-Pan film processed in Kodak developer.
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