• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Site-Specific Integration in Human ESC Using Jump-In TI Technology

        互联网

        529
        Engineering of human embryonic stem cells (hESC) offers a great potential tool for the study of human gene function. There are many techniques that can be used to engineer human cells, but most are lacking in either specificity or efficiency. Jump-In™ TI™ technology utilizes two bacteriophage recombinases (PhiC31 and R4) to specifically, efficiently, and stably introduce genetic elements into the genome of human ESCs. The techniques described here allow the user to first deliver a targeting site to a defined locus, and second to deliver the genetic elements of interest to that targeting site, allowing for stable, single copy integration into the genome. These integrated elements show high levels of expression in the pluripotent state, as well as in multiple differentiated lineages.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序