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        Use of Skin Equivalent Technology in a Wound Healing Model

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        Re-epithelialization is defined as the reconstitution of cells into an organized, stratified squamous epithelium that permanently covers a wound defect and restores function (1 ). Following wounding, keratinocytes are activated to undergo a series of phenotypic changes that have been well-characterized in vivo (2 4 ). However, in vitro studies of re-epithelialization have often been limited by their inability to simulate the in vivo tissue. Wound models using skin explants (5 8 ) or submerged keratinocyte cultures (9 ,10 ) demonstrate only partial differentiation and hyperproliferative growth. These systems have been useful for studying keratinoctye migration (11 ), but are limited in studying other aspects of re-epithelialization.
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