High-Speed Atomic Force Microscopy and Biomolecular Processes

Atomic force microscope (AFM) is unique in its capability to capture high-resolution images of biological samples in liquids. This capability will become more versatile to biological sciences if AFM additionally acquires an ability of high-speed imaging, because “direct and real-time visualization” is a straightforward and powerful means to understand biomolecular processes. However, the imaging speed of conventional AFM is too slow to capture moving protein molecules at high resolution. In order to fill this large gap, various efforts have been carried out in the past decade. In this chapter, the past efforts for increasing the scan rate and reduction of tip–sample interaction force of AFM and demonstration of direct visualization of biomolecular processes are described.