Labeling Primitive Myeloid Progenitor Cells in Xenopus

In Xenopus the first blood cells to differentiate in the embryo are the primitive myeloid lineages, which arise from the anterior ventral blood islands during the neurula stages. Primitive myeloid cells (PMCs) will give rise to the embryonic pool of neutrophils and macrophages, a highly migratory population of cells with various functions during development and tissue repair. Understanding the development and behavior of PMCs depends on our ability to label, manipulate, and image these cells. Xenopus embryos have several advantages in the study of PMCs, including a well-established fate map and the possibility of performing transplants in order to label these cells. In addition, Xenopus embryos are easy to manipulate and their external development and transparency at the tadpole stages make them amenable to imaging techniques. Here we describe two methods for labeling primitive myeloid progenitor cells during early Xenopus development.