结构基因组学

Max Haring1 Sascha Offermann2 Tanja Danker2 Ina Horst2 Christoph Peterhansel2 and Maike Stam1 1 Swammerdam Institute for Life Sciences Universiteit van Amsterdam Kruislaan 318 1098 SM Amsterdam ...

ChIP-Seq_Sample_Prep_11257047_RevA.pdf ...

ChIP Protocol-Myerslab-112607.pdf ...

Preparing Samples for.pdf

Preparing Samples for.pdf

Day 0 Block Staph A cells (Pansorbin CalBiochem 507862) Thaw 1 tube (200 μL) of Staph A cells for approximately 5 experimental ChIPs. Add 25 μL of herring sperm DNA (Promega D1815) 10 mg/ml); previous ...

(1) Remove vitelline membrane from stage 21-23 embryos (n = 300). Collect head tissues (about anterior 1/4 or 1/3 of the embryo) in 1x MBS. (2) Fix the tissues in 0.5x MBS/1% formaldehyde (4-5 ml in ...

INTRODUCTION Chromatin immunoprecipitation (ChIP) is an invaluable method for studying interactions between specific proteins or modified forms of proteins and a genomic DNA region. ChIP can be used t ...

INTRODUCTION This protocol describes the use of chromatin immunoprecipitation technology (ChIP) to analyze interactions of proteins or protein complexes with DNA in vivo. In this approach the material ...

INTRODUCTION This protocol describes the use of chromatin immunoprecipitation technology (ChIP) to analyze interactions of proteins or protein complexes with DNA in vivo. In this approach the material ...

INTRODUCTION In cells and tissues the histone proteins that constitute the nucleosomes can present multiple post-translational modifications such as lysine acetylation lysine and arginine methylation ...

INTRODUCTION In cells and tissues the histone proteins that constitute the nucleosomes can present multiple post-translational modifications such as lysine acetylation lysine and arginine methylation ...

INTRODUCTION Progress in technologies to address long-range chromosomal interactions in vivo has extensively revised concepts about different aspects of transcriptional regulation. These methods allow ...

INTRODUCTION Progress in technologies to address long-range chromosomal interactions in vivo has extensively revised concepts about different aspects of transcriptional regulation. These methods allow ...

Construction of Gene-Targeting Vectors by RecombineeringSong-Choon Lee and Pentao Liu1 Mouse Cancer Genetics Wellcome Trust Sanger Institute Hinxton Cambridge CB10 1HH United Kingdom 1Corresponding au ...

Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian EmbryosCheng Cui1 Brenda Rongish1 Charles Little1 and Rusty Lansford23 1 Department of Anatomy and Cell Biology University of Kansas M ...

Injection of dsRNA into Drosophila Embryos for RNA Interference (RNAi)Leonie Misquitta Qin Wei and Bruce M. Paterson This protocol was adapted from "Targeted Disruption of Gene Function in Drosophila ...

In Planta Transformation of ArabidopsisDetlef Weigel and Jane Glazebrook This protocol was adapted from "How to Transform Arabidopsis" Chapter 5 in Arabidopsis by Detlef Weigel and Jane Glazebrook. Co ...

Microinjection of dsRNA into Mouse Oocytes and Early EmbryosPaula Stein and Petr Svoboda This protocol was adapted from "RNAi in Mouse Oocytes and Early Embryos" contributed by Paula Stein and Petr Sv ...

Staining Whole Mouse Embryos for β-Galactosidase (lacZ) ActivityAndras Nagy Marina Gertsenstein Kristina Vintersten and Richard Behringer This protocol was adapted from "Techniques for Visualizing Gen ...